Figure 8: Effect of ammonium chloride (NH4Cl) on alpha-synuclein (asyn), MEF2D, lamp2A, and hsc70 protein expression. (a) Representative Western blot analysis of asyn, MEF2D, lamp2A, and hsc70 in SH-SY5Y cells in basal conditions and after exposure to 10 mM NH4Cl for 24 hours. The immunoreactivity of beta-actin, used as internal standard, was also shown. (b) Mean values of asyn, MEF2D, lamp2A, and hsc70 protein expression, expressed as ratio between each target and beta-actin optical density. , paired Student’s -test; versus vehicle.