Rotenone Upregulates Alpha-Synuclein and Myocyte Enhancer Factor 2D Independently from Lysosomal Degradation Inhibition

Figure 9

Effect of ammonium chloride (NH₄Cl) on intracellular localization of MEF2D. (a) Representative Western blot analysis of MEF2D in the cytosolic (C) and nuclear (N) fractions obtained from SH-SY5Y cells in basal conditions and after exposure to 5 and 10 mM NH₄Cl for 24 hours. The immunoreactivities of beta-actin, used as internal standard for the cytosol, and PARP, used as internal standard for the nuclei, were also shown. (b) Mean values of MEF2D protein expression in cytosolic and nuclear fractions, expressed as ratio to beta-actin and PARP, respectively. 𝑁=4, repeated measures ANOVA test, followed by Dunnett’s post-test; ^∗𝑃<0.05; ^∗∗𝑃<0.01 versus vehicle.

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