Figure 9: Effect of ammonium chloride (NH4Cl) on intracellular localization of MEF2D. (a) Representative Western blot analysis of MEF2D in the cytosolic (C) and nuclear (N) fractions obtained from SH-SY5Y cells in basal conditions and after exposure to 5 and 10 mM NH4Cl for 24 hours. The immunoreactivities of beta-actin, used as internal standard for the cytosol, and PARP, used as internal standard for the nuclei, were also shown. (b) Mean values of MEF2D protein expression in cytosolic and nuclear fractions, expressed as ratio to beta-actin and PARP, respectively. , repeated measures ANOVA test, followed by Dunnett’s post-test; ; versus vehicle.