Table 1: Optimum conditions used for enzymatic proteolysis of stone fish.

EnzymeBuffer (50 mM)pHTemperature (°C)

AlcalaseBorate buffer7.955
BromelainAcetate buffer4.550
FlavourzymePhosphate buffer6.550
PapainPhosphate buffer6.555
PepsinAcetate buffer3.637
TrypsinPhosphate buffer6.937