Table 2: Comparison of the kinetic parameters of the reactions catalyzed by different SHMTs.

SubstrateE. coli SHMTaSHMTa P. ingrahamii B. stearothermophilus SHMTb

(mM)

(min−1)

(mM)

(min−1)

(mM)

(s−1)

L-Threonine43d4.3d20.2f6.6fNDcNDc
L-Threo-phenylserine19d167d17.2f852fNDcNDc
L-Allo-threonine1.5e 30e 1.6f107f0.9g0.47g
L-Serine0.3e 640e0.4f555f0.9g3.9g

Reactions were carried out at 30°C
bReactions were carried out at 37°C
cNot determined
dFrom [25]
eFrom [53]
fFrom [54]
gFrom [48].