Research Article

STAT6 siRNA Matrix-Loaded Gelatin Nanocarriers: Formulation, Characterization, and Ex Vivo Proof of Concept Using Adenocarcinoma Cells

Figure 9

Cytotoxicity of the developed S6S-GNC compared to placebo, native S6S and S6S with lipofectamine on A549 lung cancer cells. The graph shows the % cell viability observed after 24 and 48 hr following treatment. Cell viability was performed using A549 (human adenocarcinoma cell line) cells in F12-K medium supplemented with 10% FBS and an antibiotic antimycotic solution of penicillin (5000 U/mL), streptomycin (0.1 mg/mL), and neomycin (0.2 mg/mL) (PSN). Cell incubation was conducted within a humidified atmosphere of 5% CO2 at a temperature of °C. The formulation and S6S lipofectamine complexes were applied as freshly prepared solutions between 0 and 15 nM concentrations. The absorbance of the formazan crystals dissolved in DMSO was read at 540 nm on a Biospek Synergy H1 plate reader. Values are represented as mean ± SD (where ).
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