BioMed Research International

Research Article

Expression of Pluripotency and Oocyte-Related Genes in Single Putative Stem Cells from Human Adult Ovarian Surface Epithelium Cultured In Vitro in the Presence of Follicular Fluid

Figure 3

Single cells analyzed on expression of genes: eighteen single primitive oocyte-like cells (OLC1–18), developed in vitro (OLC18 further developed in a parthenogenetic blastocyst-like structure) and cultured for up to one month (OLC1–8 and OLC16–18 for 15 days, OLC9–11 for 30 days, and OLC12–15 for 5 days), one mature (O MII) and one immature (O GV) oocyte (inverted microscope, Hoffman, scale bar: 10 μm), and human embryonic stem cells (hESCs) and fibroblasts (F) under a phase-contrast microscope (magnification 200x).
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