Expression of Pluripotency and Oocyte-Related Genes in Single Putative Stem Cells from Human Adult Ovarian Surface Epithelium Cultured In Vitro in the Presence of Follicular Fluid
Figure 4
Single-cell gene expression profile of all analyzed genes in primitive oocyte-like cells developed in vitro (OLCs). Comparison with human embryonic stem cells (H1 hESCs), nonfertilized oocytes from the in vitro fertilization programme (O) and fibroblasts (F161) revealed the expression of several genes related to pluripotent stem cells and oocytes. (a) Principle component analysis (PC1 versus PC2). (b) Principle component analysis (PC2 versus PC3). (c) Hierarchical clustering. (d) Heatmap clustering. Legend of analyzed cells:OLC(1–18): primitive oocyte-like cells developed in vitro; O: oocytes; H1 hESC: human embryonic stem cells of line H1 (1: one cell, 5: five cells, 10: ten cells, and 20: twenty cells); F161: fibroblasts of F161 line (1: one cell, 5: five cells, 10: ten cells, 20: twenty cells); MII: mature, metaphase II oocytes; IVM: immature, in vitro matured oocytes; MI: immature, metaphase I oocytes; GV: immature, germinal vesicle oocytes from the in vitro fertilization programme. Cell group colours: red: oocytes from the in vitro fertilization programme, green: primitive oocyte-like cells, developed in vitro, aquamarine: hESCs, and dark blue: fibroblasts.
