Expression of Pluripotency and Oocyte-Related Genes in Single Putative Stem Cells from Human Adult Ovarian Surface Epithelium Cultured In Vitro in the Presence of Follicular Fluid

Table 2

The mean concentrations of reproductive hormones in samples of culture medium (DMEM/F-12) with added follicular fluid (FF). Retrieved from two different ovarian cell cultures (OSE) with developing oocyte-like cells after 15–17 days of culturing in comparison with the same culture medium with added follicular fluid (without OSE cell culture) and the same culture medium (without added follicular fluid and OSE cell culture).

	Concentrations of hormones in culture medium
	Estradiol*; (nmol/L)	Progesterone**; (nmol/L)	Androstenedione; (nmol/L)	Testosterone; (nmol/L)
DMEM/F-12; + FF; + OSE; (min.−max.)	175.5; (149.0–202.0)	1473.5; (3999.0–1052.0)	81.4; (82.5–80.3)	2.55; (2.4–2.7)
DMEM/F-12+ FF; (min.−max.)	310.5; (377.0–244.0)	4783.5; (5528.0–4039.0)	91.4; (90.1–92.8)	4.15; (3.9–4.4)
DMEM/F-12; (min.−max.)	0.73; (0.66–0.81)	<0.64	0.015; (0.01-0.02)	1.1; (1.0–1.2)

 *Reference serum values in women. Follicular phase: 0–0.59 nmol/L; follicular phase, day 2-3: 0–0.31 nmol/L; at ovulation: 0.12–1.47 nmol/L; luteal phase: 0.1–0.9 nmol/L; nontreated postmenopausal: 0–0.11 nmol/L; treated postmenopausal: 0–0.34 nmol/L; at oral contraception: 0–0.37 nmol/L. Reference serum value in men: 0–0.21 nmol/L.
 **Reference serum values in women. Follicular phase: up to 3.6 nmol/L; follicular phase, middle: up to 3.1 nmol/L; at ovulation: 1.5–5.5 nmol/L; luteal phase: 3.0–68 nmol/L; luteal phase, middle: 19–76 nmol/L; postmenopausal: up to 3.2 nmol/L; at oral contraception: 1.1–2.9 nmol/L; pregnancy, first quarter: 29.6–106 nmol/L; pregnancy, second quarter: 93.8–159 nmol/L; pregnancy, third quarter: 264–509 nmol/L. Reference serum value in men: 0.86–2.9 nmol/L.