7,8-Dihydroxyflavone Suppresses Oxidative Stress-Induced Base Modification in DNA via Induction of the Repair Enzyme 8-Oxoguanine DNA Glycosylase-1
Figure 2
DHF induces OGG1 promoter activity and OGG1 protein expression. (a) After overnight transfection with the OGG1-promoter luciferase vector, cells were treated with 40 μM DHF for 1 h, incubated with 1 mM H2O2 for an additional 24 h, and then subjected to luciferase assays. *Significantly different from control cells (); #significantly different from H2O2-treated cells (). (b) Cells were treated with 40 μM DHF for 1 h and then incubated with 1 mM H2O2 for an additional 24 h. OGG1 mRNA levels were detected by RT-PCR analysis and (c) quantitative real-time PCR. *Significantly different from control cells (); #significantly different from H2O2-treated cells (). (d) OGG1 protein levels were detected by western blot analysis.
