7,8-Dihydroxyflavone Suppresses Oxidative Stress-Induced Base Modification in DNA via Induction of the Repair Enzyme 8-Oxoguanine DNA Glycosylase-1
Figure 3
DHF induces binding of Nrf2 to small Maf transcription factor. (a) Cells were treated with 40 μM DHF for 1 h and then incubated with 1 mM H2O2 for an additional 12 h. Nuclear extracts were electrophoresed, and nuclear Nrf2, small Maf protein, and TBP were detected using specific antibodies. (b) Confocal images of Nrf2 antibody and FITC-conjugated secondary antibody staining indicate the location of Nrf2 protein (green); DAPI staining indicates the nucleus (blue). The merged images reveal the nuclear or cytosol location of Nrf2 protein. (c) Nuclear extracts were immune-precipitated with an anti-Nrf2 antibody and subjected to western blotting using an antibody against small Maf protein.
