Comparative Proteomic Analysis of Peritoneal Dialysate from Chronic Glomerulonephritis Patients
Figure 4
Western blotting of the identified proteins in peritoneal dialysates collected from other three CGN patients at their first time (E08–E10) and after a year (M08–M10) of receiving CAPD treatment to further validate these protein candidates. The mean band density of E08–E10 and M08–M10 was calculated and used to obtain the fold change between the two groups (M/E). The fold is indicated in the bracket to the right of each set of bands. Western blotting confirmed that (a) Ig mu chain C region, fibrinogen gamma, and C-reactive protein show higher expression levels in the early-stage samples than in the middle-stage samples, while (b) Ig delta chain C region, alpha-1-antitrypsin, histidine-rich glycoprotein, apolipoprotein A-I, and serum amyloid P-component were observed to have higher levels in the dialysate after one year of CAPD treatment.
