QPLOT: A Quality Assessment Tool for Next Generation Sequencing Data

Figure 1

A subset of figures generated by QPLOT on an Illumina run. (a) Empirical base quality scores versus the scores stored in the BAM files. (b) Empirical base quality scores by cycles. (c) Bias of depth by GC content. (d) Insert size distribution.

(a) Run92Trim empirical versus reported Phred score

(b) Run92Trim empirical Phred score by cycle

(c) Run92Trim mean depth versus GC

(d) Run92Trim insert size distribution