[Retracted] MicroRNA-124 Regulates the Proliferation of Colorectal Cancer Cells by Targeting iASPP
Figure 4
Effects of miR-124 and iASPP on the viability, proliferation, and clone forming ability of SW480 or HT29 or HT29 cells. SW480 or HT29 cells were transfected with scrambled miR lentivirus (without miR-124) (miR-SRC), miR-124 lentivirus, iASPP shRNA, or control shRNA. (a) MTT cell proliferation assay. Ectopic overexpression of miR-124 using an miR-124 lentivirus or iASPP shRNA significantly reduced the proliferation of SW480 or HT29 cells, compared to NC () (b) Cell viability, as determined by the BrdU incorporation assay. (c) Colony formation assay. Cells were seeded in soft agar as described in Section 2. (d) Number of colonies at two weeks after seeding. The figure is representative of three experiments with similar results.
