Research Article

p55PIK Transcriptionally Activated by MZF1 Promotes Colorectal Cancer Cell Proliferation

Table 2

Primers for site-directed mutagenesis of construct (−1243/+45) p55PIK.

Name Sequence changeSense primer sequence (5′-3′)/antisense primer sequence (5′-3′)

RUNX1-mutACCACC to GTGTATGCAACCGGCCTCAGCCAATCGTGTATCACCTTGCCTCTCTTCCCTC
GAGGGAAGAGAGGCAAGGTGATACACGATTGGCTGAGGCCGGTTGC
MZF1-mut1TGGGGA to CTAGTGGAAGCCTAGAGAGCGGCTAGTGGACGTGAAAGTCGAG
CTCGACTTTCACGTCCACTAGCCGCTCTCTAGGCTTC
MZF1-mut2GGGGG to TAGTCCGTACCATTCTGATTGAGGCTTAGTCAGAGCGCGGATGGCTGATTGGG
CCCAATCAGCCATCCGCGCTCTGACTAAGCCTCAATCAGAATGGTACG
MZF1-mut3TGGGGA to CTAGTGCGAGAGGCGTGGCTGATCTAGTGGCCGAAGCCTAGAGAGCGG
CCGCTCTCTAGGCTTCGGCCACTAGATCAGCCACGCCTCTCG
YY1-mutACCAT to GTTGACCAGCGCCCGGATCGTGTTGATCTGATTGAGGCTGG
CCAGCCTCAATCAGATCAACACGATCCGGGCGCTGG