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BioMed Research International
Volume 2013 (2013), Article ID 875437, 6 pages
Evaluation of Multiplex PCR with Enhanced Spore Germination for Detection of Clostridium difficile from Stool Samples of the Hospitalized Patients
1Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
2Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
3Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand
4Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand
5Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand
6Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Received 23 November 2012; Revised 31 January 2013; Accepted 16 February 2013
Academic Editor: Joy Scaria
Copyright © 2013 Surang Chankhamhaengdecha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- K. C. Carroll and J. G. Bartlett, “Biology of Clostridium difficile: implications for epidemiology and diagnosis,” in Annual Review of Microbiology, vol. 65, pp. 501–521, 2011.
- N. Oezguen, T. D. Power, P. Urvil et al., “Clostridial toxins: sensing a target in a hostile gut environment,” Gut Microbes, vol. 3, no. 1, pp. 35–41, 2012.
- S. Bacci, K. Mølbak, M. K. Kjeldsen, and K. E. P. Olsen, “Binary toxin and death after Clostridium difficile infection,” Emerging Infectious Diseases, vol. 17, no. 6, pp. 976–982, 2011.
- C. Schwan, B. Stecher, T. Tzivelekidis et al., “Clostridium difficile toxin CDT induces formation of microtubule-based protrusions and increases adherence of bacteria,” PLoS Pathogens, vol. 5, no. 10, article e1000626, 2009.
- S. T. Cartman, J. T. Heap, S. A. Kuehne, A. Cockayne, and N. P. Minton, “The emergence of 'hypervirulence' in Clostridium difficile,” International Journal of Medical Microbiology, vol. 300, no. 6, pp. 387–395, 2010.
- E. J. Kuijper, R. J. van den Berg, and J. S. Brazier, “Comparison of molecular typing methods applied to Clostridium difficile,” Methods in Molecular Biology, vol. 551, pp. 159–171, 2009.
- M. Delmée, J. Van Broeck, A. Simon, M. Janssens, and V. Avesani, “Laboratory diagnosis of Clostridium difficile-associated diarrhoea: a plea for culture,” Journal of Medical Microbiology, vol. 54, part 2, pp. 187–191, 2005.
- L. R. Peterson, P. J. Kelly, and H. A. Nordbrock, “Role of culture and toxin detection in laboratory testing for diagnosis of Clostridium difficile-associated diarrhea,” European Journal of Clinical Microbiology and Infectious Diseases, vol. 15, no. 4, pp. 330–336, 1996.
- H. Snell, M. Ramos, S. Longo, M. John, and Z. Hussain, “Performance of the TechLab C. DIFF CHEK-60 enzyme immunoassay (EIA) in combination with the C. difficile tox A/B II EIA kit, the triage C. difficile panel immunoassay, and a cytotoxin assay for diagnosis of Clostridium difficile-associated diarrhea,” Journal of Clinical Microbiology, vol. 42, no. 10, pp. 4863–4865, 2004.
- H. Vanpoucke, T. De Baere, G. Claeys, M. Vaneechoutte, and G. Verschraegen, “Evaluation of six commercial assays for the rapid detection of Clostridium difficile toxin and/or antigen in stool specimens,” Clinical Microbiology and Infection, vol. 7, no. 2, pp. 55–64, 2001.
- B. M. Shin, Y. K. Eun, J. L. Eun, and J. G. Songer, “Algorithm combining toxin immunoassay and stool culture for diagnosis of Clostridium difficile infection,” Journal of Clinical Microbiology, vol. 47, no. 9, pp. 2952–2956, 2009.
- E. J. Kvach, D. Ferguson, P. F. Riska, and M. L. Landry, “Comparison of BD GeneOhm Cdiff real-time PCR assay with a two-step algorithm and a toxin A/B enzyme-linked immunosorbent assay for diagnosis of toxigenic Clostridium difficile infection,” Journal of Clinical Microbiology, vol. 48, no. 1, pp. 109–114, 2010.
- M. E. Reller, C. A. Lema, T. M. Perl et al., “Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile,” Journal of Clinical Microbiology, vol. 45, no. 11, pp. 3601–3605, 2007.
- F. C. Tenover, S. Novak-Weekley, C. W. Woods et al., “Impact of strain type on detection of toxigenic Clostridium difficile: comparison of molecular diagnostic and enzyme immunoassay approaches,” Journal of Clinical Microbiology, vol. 48, no. 10, pp. 3719–3724, 2010.
- S. M. Novak-Weekley, E. M. Marlowe, J. M. Miller et al., “Clostridium difficile testing in the clinical laboratory by use of multiple testing algorithms,” Journal of Clinical Microbiology, vol. 48, no. 3, pp. 889–893, 2010.
- J. A. Barkin, N. Nandi, N. Miller, A. Grace, J. S. Barkin, and D. A. Sussman, “Superiority of the DNA amplification assay for the diagnosis of C. difficile infection: a clinical comparison of fecal tests,” Digestive Diseases and Sciences, vol. 57, no. 10, pp. 2592–2599, 2012.
- K. C. Carroll, “Tests for the diagnosis of Clostridium difficile infection: the next generation,” Anaerobe, vol. 17, no. 4, pp. 170–174, 2011.
- D. Heeg, D. A. Burns, S. T. Cartman, and N. P. Minton, “Spores of Clostridium difficile clinical isolates display a diverse germination response to bile salts,” PLoS One, vol. 7, no. 2, article e32381, 2012.
- M. Rupnik, “Heterogeneity of large clostridial toxins: importance of Clostridium difficile toxinotypes,” FEMS Microbiology Reviews, vol. 32, no. 3, pp. 541–555, 2008.
- F. Barbut, M. Monot, A. Rousseau et al., “Rapid diagnosis of Clostridium difficile infection by multiplex real-time PCR,” European Journal of Clinical Microbiology & Infectious Diseases, vol. 30, no. 10, pp. 1279–1285, 2011.
- R. A. Luna, B. L. Boyanton Jr., S. Mehta et al., “Rapid stool-based diagnosis of Clostridium difficile infection by real-time PCR in a children's hospital,” Journal of Clinical Microbiology, vol. 49, no. 3, pp. 851–857, 2011.
- S. D. Bélanger, M. Boissinot, N. Clairoux, F. J. Picard, and M. G. Bergeron, “Rapid detection of Clostridium difficile in feces by real-time PCR,” Journal of Clinical Microbiology, vol. 41, no. 2, pp. 730–734, 2003.
- P. Lantz, W. Abu Al-Soud, R. Knutsson, B. Hahn-Hagerdal, and P. Rådstrom, “Biotechnical use of polymerase chain reaction for microbiological analysis of biological samples,” Biotechnology Annual Review, vol. 5, pp. 87–130, 2000.
- S. Wongwanich, S. Rugdeekha, P. Pongpech, and C. Dhiraputra, “Detection of Clostridium difficile toxin A and B genes from stool samples of Thai diarrheal patients by polymerase chain reaction technique,” Journal of the Medical Association of Thailand, vol. 86, no. 10, pp. 970–975, 2003.
- D. Chotiprasitsakul, T. Janvilisri, S. Kiertiburanakul et al., “A superior test for diagnosis of Clostridium difficile-associated diarrhea in resource-limited settings,” Japanese Journal of Infectious Diseases, vol. 65, pp. 326–329, 2012.