Evaluation of Multiplex PCR with Enhanced Spore Germination for Detection of Clostridium difficile from Stool Samples of the Hospitalized Patients

Table 1

Primers in the multiplex PCR for the detection of C. difficile.

Target	Primer	Sequence (5^′-3^′)	Primer concentration (𝜇M)	Amplicon size (bp)
tcdA	tcdA-F	GTATGGATAGGTGGAGAAGTCAGTG	0.025
	tcdA-R	CGGTCTAGTCCAATAGAGCTAGGTC	0.025	632
tcdB	tcdB-F	GAAGATTTAGGAAATGAAGAAGGTGA	0.01
	tcdB-R	AACCACTATATTCAACTGCTTGTCC	0.01	441
cdtA	cdtA-F	ATGCACAAGACTTACAAAGCTATAGTG	0.2
	cdtA-R	CGAGAATTTGCTTCTATTTGATAATC	0.2	260
cdtB	cdtB-F	ATTGGCAATAATCTATCTCCTGGA	0.5
	cdtB-R	CCAAAATTTCCACTTACTTGTGTTG	0.5	179
16s rDNA	UFU-L	GCCTAACACATGCAAGTCGA	0.025
	UR802	TACCAGGGTATCTAATCC	0.025	800