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BioMed Research International
Volume 2013 (2013), Article ID 875958, 11 pages
http://dx.doi.org/10.1155/2013/875958
Research Article

Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells

1Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 07360 Mexico, DF, Mexico
2Coordinación Academica, Universidad Autónoma de la Ciudad de México, 06720 Mexico, DF, Mexico
3Department of Biochemestry, Faculty of Medicine, Universidad Nacional Autonoma de México, Edificio de Investigación, 04510 Mexico, DF, Mexico
4Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-0015, USA

Received 8 April 2013; Revised 19 September 2013; Accepted 25 September 2013

Academic Editor: Vittorio Sambri

Copyright © 2013 Maria de Lourdes Muñoz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The main vector of dengue in America is the mosquito Aedes aegypti, which is infected by dengue virus (DENV) through receptors of midgut epithelial cells. The envelope protein (E) of dengue virus binds to receptors present on the host cells through its domain III that has been primarily recognized to bind cell receptors. In order to identify potential receptors, proteins from mosquito midgut tissue and C6/36 cells were purified by affinity using columns with the recombinant E protein domain III (rE-DIII) or DENV particles bound covalently to Sepharose 4B to compare and evaluate their performance to bind proteins including putative receptors from female mosquitoes of Ae. aegypti. To determine their identity mass spectrometric analysis of purified proteins separated by polyacrylamide gel electrophoresis was performed. Our results indicate that both viral particles and rE-DIII bound proteins with the same apparent molecular weights of 57 and 67 kDa. In addition, viral particles bound high molecular weight proteins. Purified proteins identified were enolase, beta-adrenergic receptor kinase (beta-ARK), translation elongation factor EF-1 alpha/Tu, and cadherin.