BioMed Research International

Research Article

Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells

Table 2

Distinct host peptides identified by mass spectrometry bound to DENV.


Cell/tissue expression	Protein name	Experment number	Peptide identified	Score

C6/36 (57 kDa)	Enolase	1	K.EALNLIQDAIAK.A	45.6
			R.GNPTVEVDLVTDLGLFR.A	62.1
			K.VNQIGTVTESINAHLLAK.K	76.1
			R.SGETEDTFIADLVVGLSTGQIK.T	76.1
C6/36 (67 kDa)		2	FGLDATAVGDEGGFAPNILNNKEALDLINEAISK	60.5
DS3		3	GVLKAVTQ	20.2
DMEB (67 kDa)		4	R.AAVPSGASTGVHEALELR.D	53.2
DMEB (67 kDa)		4	K.NLILPVPAFNVINGGSHAGNKQAMQEFMILPTGACSFTEAMK.M	21.7

DMEB (67 kDa)	Beta-adrenergic receptor kinase	1	ESQELLGSMAKK	40.1
DS3 (67 kDa)		2	ESQELLGSMAKK	40.1
C6/36 (67 kDa)		3	AKPGAEAHPPFRQHK	26.9

C6/36 (57 kDa)	Translation elongation factor EF-1 alpha/Tu	1	SGDAAIVNLVPSWPLCVESFQEFPPLGR	82.9
Mori (extract)		2	NNPPKQAA	21.8
IBO		3	K.GASDFTAQVIVLNHPGQIANGYTPVLDCHTAVIACKFAEIQQK.V R.LPLQDVYK.I	63

C6/36 (80 kDa)	Cadherin	1	FLIDYGSGTLELRIATK	52

Proteomic analysis was performed in protein from C6/36 cells, mosquito MGS purified by affinity chromatography (extract), or in the bands of interest excised after separation by SDS-PAGE.