Table 1: Experimental study design.

GroupsGroups of mice
(nos. of mice)
PrimeAnalyses after the prime (compartment)Boost (time after prime)Analyses after the boost (compartment)Challenge (time after prime)Time of analyses after challenge (compartment)

Experiment (Figures 1(a) and 1(b))
Group 1Young (15)AdC68NPWks 2, 4, 6, and 8
(blood)
 NoneWks 2, 4, 6, 8
(blood)
A/PR8 (mo 4)Day 20
(blood)
Group 2Aged (13)
Group 3Young (4)A/X31 (mo 2)
Group 4Aged (13)
Group 5Young (5) None
Group 6Aged (10)
Group 7Young (25) None
Group 8Aged (33)

Experiment (Figures 2(a), 2(b), and 2(c))
Group 1Young (5)AdC68NPNoneWk 8
(blood, spleen, and lung)
A/PR8 (mo 4)Days 5, 20
(blood, spleen, and lung)
Group 2Aged (8)
Group 3Young (5)A/X31 (mo 2)
Group 4Aged (5) None
Group 5Young (5) None
Group 6Aged (5)
Group 7Young (2)None
Group 8Aged (4)

Experiment (Figures 3(a) and 3(b))
Group 1Young (4)AdC68NPNoneA/PR8 (mo 4)Day 20
(spleen)
Group 2Aged (4)
Group 3Young (4)A/X31 (mo 2)
Group 4Young (5) NoneNoneNone
Group 5Aged (4)
Group 6Young (5)None
Group 7Aged (3)

Experiment (Figures 4(a), 4(b), 4(c), and 4(e))1
Group 1Young (15) 5 AdC68NPNoneA/PR8 (mo 4)Checked daily for weight loss and survival;
viral titers in lungs tested on day 5;
lung infiltrates tested on day 5.
Group 2Aged (13) [15 7
Group 3Young (4 5 A/X31 (mo 2)2
Group 4Aged (8) 5 5 None None
Group 5Young 5 5  None
Group 6Aged (10) 7 4
Group 7Young (25) 9 None
Group 8Aged (38) 7

Number of mice tested for survival are shown in (), numbers of mice tested for viral loads are shown in regular font in , and numbers of mice used to determine subsets of lung infiltrating cells are shown on bold italic in .
2Some of the mice were primed at a young age. They were boosted ~18 months later.