Figure 5: Morphometric comparisons of control and ligated bone-PDL-cementum complexes, including changes in collagen birefringence. (a) Comparisons of measurements in mesial and distal regions of the CEJ-ABC (i), the interradicular region (ii), and the PDL-space (iii, iv) between control and ligated complexes at 4, 8, and 15 days. Individual graphs were used to compare PDL-space measurements between coronal and apical anatomical locations within mesial (iii) and distal (iv) complexes. (b) The differences between average ligated and average control PDL-space measurements are plotted for each aforementioned anatomical location across time. (c) Histological sections show the distal complex stained with PSR and visualized under polarized light microscopy. Alveolar bone (AB), periodontal ligament (PDL), secondary cementum (SC), and transseptal fibers (TF). Symbols within plots indicate statistically significant differences at 95% confidence interval. Significant difference between control and ligated groups. Significant difference over time. Significant difference between control and ligated groups. *Significant difference over time. Significant difference between control and ligated groups. ▲,∆Significant difference over time.