Reproducibility of NMR Analysis of Urine Samples: Impact of Sample Preparation, Storage Conditions, and Animal Health Status
Figure 2
1H NMR spectra of rat urine after treatment with natrosol, furosemide, or HCBD. Rats were treated with natrosol, furosemide, or HCBD and urine was collected at 0–8 h for furosemide and at 8–24 h for natrosol and HCBD to achieve pharmacological effects. Typical 1D 1H NMR NOESY spectra are shown from rat urine after treatment. All spectra were recorded under standard conditions for single metabolite quantification and plotted with an identical scale. Abbreviations: C: citrate; CA: creatinine; DSS: 4,4-dimethyl-4-silapentane-1-sulfonic acid; G: glucose; L: lactate; U: urea.
