Reproducibility of NMR Analysis of Urine Samples: Impact of Sample Preparation, Storage Conditions, and Animal Health Status
Figure 7
Effects of microbial contamination. Citrate and lactate were measured in urine samples from natrosol-, furosemide-, or HCBD-treated rats by 1H NMR metabolite quantification with or without modifications of pH or salt (stability test dataset, ). Samples were measured during storage at 4°C over a period of 49 days. A selection of samples showing typical effects on citrate and lactate from possible microbial degradation processes is plotted (circle) in comparison with not contaminated control samples (cross), and fitted with a non-linear curve fit. The colour of the symbols indicates animal treatment (natrosol, furosemide, and HCBD).
