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BioMed Research International
Volume 2013 (2013), Article ID 878374, 19 pages
http://dx.doi.org/10.1155/2013/878374
Research Article

Reproducibility of NMR Analysis of Urine Samples: Impact of Sample Preparation, Storage Conditions, and Animal Health Status

1numares GROUP, Josef Engert Straße 9, 93053 Regensburg, Germany
2Institute of Biophysics and Physical Biochemistry and Centre of Magnetic Resonance in Chemistry and Biomedicine, University of Regensburg, 93040 Regensburg, Germany
3Department of Computer Science, Technical University Dortmund, Otto-Hahn-Straße 16, 44221 Dortmund, Germany
4Group General Pharmacology, Department Drug Discovery Support, Boehringer-Ingelheim Pharma GmbH & Co. KG, 88397 Biberach an der Riss, Germany

Received 25 February 2013; Revised 21 May 2013; Accepted 22 May 2013

Academic Editor: Anita M. Oberbauer

Copyright © 2013 Christina Schreier et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary material provided includes:

(1) A table giving the measured pH values of all stability test samples before and after buffering. The table also includes the dilution factor resulting from the addition of HCl or NaOH.

(2) A table giving the salt concentrations and measured osmolality of all stability test samples. The table also includes the dilution factor resulting from the addition of the concentrated NaCl-solution.

(3) A histogram depicting glucose, creatinine and urea concentrations in rat urine after treatment of rats with vehicle or reference compounds, i.e. natrosol, furosemide, formoterol, spironolactone, amiloride, acetaminophen, adriamycine or hydrochlorothiazide.

(4) A histogram depicting sodium and chloride concentrations, pH and osmolality in rat urine after treatment of rats with vehicle or reference compounds, i.e. natrosol, furosemide, formoterol, spironolactone, amiloride, acetaminophen, adriamycine or hydrochlorothiazide.

  1. Supplementary Materials