Table 1: Overview of samples used for the various experiments of this study.

Sample nameSourceCharacteristicsUse

Calibration Synthetic10, 100, 1,000, 10,000 µg/mLCalibration for single metabolite quantification
Accuracy test SyntheticDefined composition (mixture of 4 metabolites at different concentrations) in a range of 10 to 10,000 µg/mLDetermination of accuracy of measurement
Stability test Treatment-wise pooled urine samples (urine from 5 male and 5 female rats per pooled sample)Treatment with natrosol, furosemide, or HCBDDetermination of effects from
(i) pH and salinity
(ii) Storage (refrigerating, freezing)
(iii) Drug treatment
Freeze-thaw testTreatment-wise pooled urine samples (urine from 2 male rats per pooled sample)Treatment with natrosol, furosemide, or HCBD Determination of effects from multiple freeze-thaw cycles
Biological variance test Urine samples from individual male or female ratsTreatment with natrosol, furosemide, or HCBDDetermination of interindividual biological variance and effects from sample composition on interindividual variance