Evaluation of a Thiolated Chitosan Scaffold for Local Delivery of BMP-2 for Osteogenic Differentiation and Ectopic Bone Formation
Figure 1
Physicochemical properties of Thio-CS. (a) Photograph of lyophilized CS and Thio-CS (left panel) and its SEM morphologies (right panel, 100x), (b) determination of free thiol or disulfide bond according to the air-oxidation time, (c) influence of the pH on the formation of disulfide bonds, and (d) effect of Thio-CS containing BMP-2 on swelling. The amount of the thiol groups in the supernatant or the pellet after centrifugation was determined spectrophotomertically using Ellmans’s reagent to quantify the free thiol groups, as described in Section 2. The values indicated are means ± SD ().
