Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human Apolipoprotein A5 Gene Promoter

Figure 3

Identification of the response sequence to CREBH in the human APOA5 gene promoter. A series of deletion constructs of human APOA5 promoter luciferase reporters and a construct containing a mutation (cross) of the putative CREBH binding site (CREBHRE) were cotransfected with a plasmid expressing CREBH-N or the empty plasmid pcDNA3 as a control, into HepG2 cells. Luciferase activity was measured using the Dual-Glo Luciferase Assay System. Representative data from three independent experiments are shown. Statistical differences from controls are indicated by asterisks (^∗𝑃<0.05 and ^∗∗𝑃<0.01).