895706.fig.002a
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895706.fig.002b
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895706.fig.002c
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Figure 2: Regulation of the human osteocalcin enhancer/promoter by vitamins D3 and D2 in vivo. (a) Enhanced expression of the OC-Luc transgene by 1α,25(OH)2D3 treatment. OC-Luc Tg mice were given a single oral administration of previtamin D3, 1α,25(OH)2D3, or vehicle. In vivo bioluminescence imaging was performed at 6 h after the administration. (b) Response of the OC-Luc transgene to 1α,25(OH)2D2 treatment. OC-Luc Tg mice were given a single oral administration of previtamin D2, 1α,25(OH)2D2, or vehicle. In vivo bioluminescence imaging was performed at 6 h after the administration. (c) Time course of the induction of the OC-Luc transgene by vitamin D treatment. OC-Luc Tg mice were given a single oral administration of 1α,25(OH)2D3 (filled circles), 1α,25(OH)2D2 (shaded circles), or vehicle (open circles). The bioluminescence was analyzed at 0, 6, 9, and 24 h after the administration using Living Image software and represented as the ratio of total flux (photons/second) compared to 0 h. The data shown are means ± SE ( ). Statistical analysis was performed by Student's t-test; * relative to vehicle.