About this Journal Submit a Manuscript Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 898719, 7 pages
http://dx.doi.org/10.1155/2013/898719
Research Article

Impact of the 2009 (7th Edition) AJCC Melanoma Staging System in the Classification of Thin Cutaneous Melanomas

1Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA
2Section of Dermatopathology, Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
3Department of Biostatistics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
4Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA

Received 30 September 2013; Revised 23 October 2013; Accepted 28 October 2013

Academic Editor: Giuseppe Argenziano

Copyright © 2013 Vicki H. Chu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Context. The 7th (2009) edition of the AJCC melanoma staging system incorporates tumor (Breslow) thickness, MR, and ulceration in stratifying T1 primary melanomas. Compared to the prior 6th (2001) edition, MR has replaced CL for thin melanomas. Objective. We sought to identify and report differences of the classification of thin melanomas as well as outcome of SLNB in patients according to the 6th and 7th editions at our institution. Results. 106 patients were identified with thin melanomas verified by wide excision. 31 of 106 thin melanomas were reclassified according to the 7th edition of the AJCC. Of those 31, 15 CL II/III patients (6th edition T1a) were reclassified as T1b based on the presence of mitoses while 16 CL IV patients (6th edition T1b) were categorized as T1a based on the absence of mitoses. 26/31 reclassified patients underwent SLNB, and all were negative. Patients with thin melanoma and a +SLNB ( ) were all classified as T1b according to both staging systems. Conclusions. In our experience, 29% of thin melanomas were reclassified according to the 7th edition with similar proportions of patients re-distributed as T1a (14%) and T1b (15%). Cases with +SLN corresponded with T1b lesions in both 6th and 7th editions.

1. Introduction

Primary cutaneous melanoma is a serious type of skin malignancy and accounts for the majority of skin cancer deaths. The incidence of cutaneous melanoma has risen steadily and in the United States alone has increased at an annual rate of 3.1% over the past several decades [1]. The estimated lifetime risk of an American developing invasive melanoma is 1 in 59 and is projected to rise to 1 in 50 by the year 2015 [2]. Increased surveillance and enhanced public awareness have contributed to early detection of thin melanomas and have likely contributed to the increased incidence of melanoma [3]. The incidence rates for both thick and thin melanoma continue to increase for individuals 65 years or older and [4] thin melanomas account for 30% of total melanoma deaths. These features underscore the biologic significance of these tumors and the importance of wide excision and the need to delineate the subset of thin melanomas with the capacity for a more aggressive clinical course [4].

The tumor-node-metastasis (TNM) classification of melanoma defines the stage of disease and guides patient management decisions. The staging and prognosis of cutaneous melanoma continue to be refined; the most recent version was published as 2009 (7th edition) AJCC melanoma staging system. Breslow tumor thickness (BT) is one of the most important predictors of prognosis for invasive primary cutaneous melanoma [58]. Historically, the depth of tumor invasion has been described in terms of both Clark level (CL) and BT. CL defines the anatomic compartment of the skin involved by melanoma cells, whereas BT represents the maximum vertical thickness of the tumor measured from the top of the granular cell layer to the deepest invasive component [9, 10]. Thin melanomas as defined by the American Joint Committee on Cancer (AJCC) melanoma staging system are invasive lesions with BT ≤ 1.0 mm [7]. Thin melanomas account for approximately 70% of cutaneous melanoma diagnoses and are overall associated with a favorable prognosis [11, 12]. The ten-year melanoma specific survival for thin melanomas ranges from 97 to 82% depending on BT, mitosis, and ulceration [13]. Therefore, a subset of thin melanomas has a risk for metastasis and death [7, 14].

Historically, both CL and BT were used to define T categories, while primary tumor ulceration was incorporated into the staging system later [6]. Tumor ulceration is a poor prognostic indicator, and ulcerated melanomas were upstaged beginning with AJCC revisions in 2001. The 2001 AJCC melanoma staging system used CL and ulceration to subclassify thin melanomas as T1a or T1b. Primary tumors with CL IV or V or with ulceration were staged as T1b whereas lesions with CL < IV and no ulceration were staged as T1a [6]. Analyses culminating in the 7th edition of the AJCC melanoma staging database identified primary tumor BT, ulceration, and mitotic rate (MR) as the dominant independent predictors of survival in the histopathologic stratification of thin cutaneous melanomas [7, 13]. Significantly, MR was determined to be a stronger predictor of melanoma specific survival of thin melanomas when compared with CL; therefore, MR has replaced CL in the subclassification of T1 melanomas according to the 2009 AJCC staging and classification system [7, 1517]. The presence of primary tumor ulceration continues to define T1b melanomas [7].

Regional nodal tumor burden is another important prognostic factor of survival for patients with invasive cutaneous melanoma. Lymphatic mapping and sentinel lymph node biopsy (SLNB) facilitate the evaluation and identification of nodal disease [1820]. The sentinel lymph node(s) (SLN) is the first lymph node(s) to receive lymphatic drainage from a tumor at a particular anatomic area [21]. Thus, that SLN is the most likely regional lymph node to contain a metastatic deposit and functions as the best surrogate of nodal disease burden. Overall, the incidence of SLN metastasis for thin melanomas is typically low; however, there is a subset of thin melanomas that demonstrates at least a 5% risk for microscopic nodal disease [22].

We sought to identify a group of patients whose melanomas were evaluated after the implementation of 2009 AJCC staging system and examine the impact of the revised melanoma staging system in stratifying T1a and T1b lesions in patients with confirmed thin melanomas verified by examination of wide excision specimens. The outcome of SLNB, when performed, was also collected among this subset of patients with thin melanomas.

2. Material and Methods

The surgical pathology database at a major cancer center was searched for invasive cutaneous melanomas from January 1, 2010, to June 30, 2011. This study was approved by the institutions review board. Patients with biopsies were reviewed by at least one of the members of the dermatopathology faculty (MTT, VGP, CAT, MSM, DI, WLW, and JLC) and included all reported histologic parameters in a melanoma pathology template, specifically including Clark level (CL), Breslow thickness (BT), mitotic rate (MR), and ulceration (U). In addition, SLN status was collected in a subset of patients with verified thin melanomas who had this procedure as part of their management.

Mitotic rate was assessed in the invasive melanoma by examination of hematoxylin and eosin (H&E) stained sections. The number of sections varied depending on whether they were specimens processed at MDACC or were referred from other institutions. At any rate, the evaluation included all sections of routine H&E levels that were available. Histologically acceptable mitotic figures included tumors with condensed chromatin in metaphase, anaphase, or telophase of the mitotic cell cycle. The initial mitotic count, reported as number of mitotic figures/mm2, began in the area of invasive tumor with the highest number of mitotic figures or mitotic “hot spot” and examination of successive microscopic fields (4.5 h.p.f at 400x magnification in our Olympus BH-50) until one mm2 of tumor had been examined. In thin melanomas with less than one mm2 area of invasive tumor, the presence of at least one mitotic figure in any field (i.e., hot spot) constituted a mitotic rate of 1/mm2 [8, 23, 24]. Of the 327 patients identified with invasive cutaneous melanoma, 138 patients had reported BT ≤ 1.0 mm. Of these, 106 patients had wide excision available for review and confirmed BT ≤ 1.0 mm. Patients without wide excision verification of initial biopsy of thin melanomas ( ) and patients with residual tumors with BT > 1.0 mm in wide excision specimens ( ) were excluded from the final analysis. Patients with residual tumor and CL or BT greater than initially reported were used in the final analysis as long as each reported BT ≤ 1.0 mm. Mitotic rate reported as <1 mitosis/mm2 or “not applicable” (i.e., due to a very small dermal component) was considered to be equivalent to 0/mm2. Thin melanomas verified by examination of wide excision were classified as T1a and T1b according to both the 2001 and 2009 AJCC staging systems.

A number of patients with wide excision-verified T1 lesions also underwent SLNB. The protocol for histologic evaluation of SLNB at MDACC involved initial examination of H&E stained sections of SLN bread-loafed perpendicular to the long axis and has been described elsewhere [25, 26]. If the SLN was negative on the initial H&E section, a second H&E section (approximately 200 microns deeper into the tissue block) and an additional section submitted for immunohistochemical analysis with panmelanocytic cocktail (includes antibodies to detect HMB45, Mart-1, and tyrosinase) were reviewed to evaluate for the presence of SLN metastasis [26, 27].

Continuous variables of BT and number of mitoses/mm2 were compared among groups by using the Wilcoxon rank-sum test. Categorical variables of regression and the presence of an associated nevus were compared between groups by using Fisher exact test. No adjustment was made for the multiplicity of testing.

3. Results

Classification of thin melanomas among group of patients whose melanomas were evaluated after the modification of 2009 AJCC melanoma staging systems was compared with 2001 version and is presented in Table 1. The male to female ratio was 1.2 : 1, with age ranging from 11 to 86 years (median 56.5 years). The median BT of each group ranged from 0.30 to 0.90 mm. A total of 31/106 (29%) T1 lesions were reclassified when compared to the 2001 and 2009 AJCC staging guidelines. Sixteen patients with nonulcerated tumors that were CL IV and no mitotic figures were reclassified from T1b in the 2001 AJCC melanoma staging system to T1a according to the 2009 AJCC melanoma staging system. In addition, fifteen nonulcerated T1 lesions with CL II ( ) or III ( ) that were defined as T1a under the 2001 melanoma staging system were reclassified to T1b based on the presence of at least one mitotic figure/mm2 according to the 2009 AJCC melanoma staging system. Forty-five (42.5%) and 30 (28.3%) of the 106 thin melanomas retained their classification as T1a and T1b lesions according to both 2001 and 2009 AJCC melanoma staging systems, respectively. Overall, a net increase of a single T1a patient (with a commensurate decrease in 1 T1b patient) was noted when the melanomas were reclassified according to the 2009 AJCC melanoma staging system among these patients.

tab1
Table 1: Classification of patients with T1 invasive melanoma according to the 2001 and 2009 AJCC staging and classification system .

Thin melanomas were further classified according to CL, number of mitoses/mm2, ulceration, and, among those patients who had the procedure, status of SLNB. The median BT of 0.30 mm in T1a lesions with CL II, absence of mitosis, ulceration, and negative SLNB was significantly lower ( ) when compared to all other reclassified patients groups. In the 31 patients whose T-stage was reclassified the median BT ranged from 0.55 mm to 0.79 mm and was not significantly different between the sixteen patients (CL IV, no mitotic figures, no ulceration) previously categorized as T1b and reclassified to T1a when compared to the fifteen patients (CL II or III, with any mitotic rate, no ulceration) previously categorized as T1a and now re-classified as T1b based on the presence of at least one mitosis/mm2. The median mitotic rates of the reclassified T1b patients with CL II ( ) and CL III ( ) were each 1/mm2. The category with the greatest median BT (0.90 mm) was CL III with MR > 1 and ulceration ( ). This group also had the highest median mitotic rate of 3/mm2; however, SLNB were all negative (0/3). Tumor ulceration was detected in 3.8% (4/106) of the T1 lesions. All patients with ulcerated tumors ( ) had SLNB, and all of these were negative (0/4).

The three patients with positive SLNB were classified as T1b under both the 2001 and 2009 AJCC melanoma staging systems and had a median BT of 0.81 mm and a median MR of 2/mm2. All three patients had CL IV tumors with absence of ulceration and regression and no associated nevus. Deep margin involvement on initial biopsy exam was absent in this cohort. In this cohort of T1b patients with positive SLNB, the average BT and MR were not significantly different when compared to the 15 reclassified T1b patients with negative SLNB.

The clinical and histopathologic characteristics of these three patients with positive SLNB are shown in Table 2 and a representative example of thin melanoma and positive SLNB in Figure 1. Tumor infiltrating lymphocyte (TIL) response, satellitosis, and perineural invasion (PNI) were also considered for analysis; however, nearly all measurements were categorized as nonbrisk (TIL) and without satellitosis or PNI. Thus, these parameters were not subsequently analyzed. The presence of regression or an associated nevus between groups was not statistically significant. Each positive SLNB case demonstrated microscopic foci of tumor with <10 cells detected by immunohistochemistry only (Figure 1). Completion lymph node dissections (CLND) were negative in all of these patients.

tab2
Table 2: Clinical and histopathologic characteristics of the three T1 patients with positive SLN.
fig1
Figure 1: Representative case of thin melanoma ((a), (b)) with dermal mitosis ((c), arrow) and corresponding SLNB (d) with isolated HMB45+ melanoma cell (e) hematoxylin-eosin stain; original magnifications: ×1 (a); ×20 (b); ×40 ((c), (d), (e)).

The percent of SLNB performed for thin melanomas is shown in Table 3. SLNB was performed in 61.3% (65/106) of patients with thin melanomas in this cohort. Of these, 26.2% (17/65) were lesions designated with the descriptor of “at least” regarding BT, since tumor cells involved the base of the shave biopsy specimen. Patients with T1 melanomas that had the same subclassification under both the 2001 and 2009 AJCC melanoma staging system had SLNB at a rate of 31% for T1a and 87% for T1b. Among the T1b patients, 3 had positive SLNB with microscopic tumor deposits (fewer than 10 tumor cells detected in SLN). 83% (26/31) of patients with reclassified T1a and T1b tumors had SLNB and all were negative. In this study, patients with positive SLNB had BT of at least 0.54 mm, whereas all patients with BT < 0.54 mm had negative SLNB.

tab3
Table 3: Staging and rate of SLNB in reported cohorts of patients with thin melanomas.

4. Discussion

Early detection of thin melanomas remains an essential factor for long-term survival since melanomas less than 1.0 mm in thickness have a good prognosis and predicted 10-year survival of up to 97%. While surgical excision may be curative in greater than 90% of patients with thin melanomas (T1 lesions), there is a subset of patients who may ultimately develop aggressive clinical behavior.

Staging of cutaneous melanoma continues to evolve as risk factors are identified to be associated with poor or favorable survival. In analyses leading to its incorporation into the 2009 AJCC melanoma staging system, mitotic rate was determined to be an important histologic parameter in evaluating thin melanomas. Mitotic rate was an independent adverse predictor of survival and ultimately replaced Clark level to define T1b lesions [7, 13, 15]. MR ≥ 1/mm2 and BT > 0.76 mm are worrisome histological parameters for micrometastasis in a subset of patients with thin melanomas; however, the criteria for selecting “high-risk” thin melanomas for SLNB continue to evolve [2831].

Mitotic rate is determined by the number of mitoses per millimeter squared (mm2) in the invasive melanoma component and all sections on the slides should be examined to evaluate for mitoses or “hot spot.” In thin melanomas that comprise less than a one mm2 (or less than 4.5 h.p.f at 400x magnification) area of invasive tumor available for examination, the presence of mitoses in any field (hot spot) is constituted as a mitotic rate ≥1/mm2. MR should not be averaged or reported as a fraction (e.g. 0.5/mm2), and mitoses of intraepidermal melanocytes should not be included in the MR. Standard clinical practice is to interpret MR of <1/mm2 or “not applicable” due to small dermal component as MR = 0/mm2. Additional levels and/or depletion of tissue block in search for mitoses should be avoided.

This study was conducted to evaluate the differences in the distribution of T1a and T1b melanomas according to the 2001 and 2009 AJCC melanoma staging system. In our study, 29% of the T1 melanomas were reclassified. According to the 2009 AJCC melanoma staging system, there were a greater number of T1a melanomas (61/106 or 57.5%) compared with T1b melanomas (45/106 or 42.4%); however, the percentage of T1a and T1b melanomas was similar by both 2001 and 2009 AJCC melanoma staging system. SLNB was performed in 83.9% (26/31) of patients reclassified as T1a and T1b—all of which had negative SLNB. In our study, the 2009 AJCC melanoma staging system did not identify additional T1b thin melanomas with positive SLNB that would have been classified as T1a under the 2001 staging system. All patients who had a positive SLNB were classified as T1b under both the 2001 and 2009 AJCC melanoma staging systems.

The risk of SLN metastasis for thin melanomas has been reported to vary from 1.1% to 16.0% [22, 32]. Historically (i.e., prior to the 6th edition AJCC staging system), “thin melanomas” were defined as tumors with BT < 0.75 mm. In one study, thin melanomas with BT ≥ 0.75 mm had a greater than 5.0% risk for SLN metastasis, while melanomas <0.75 mm had a 2.7% risk for SLN metastasis [33]. Another study reported a progressive increase in rates of positive SLNB for invasive melanomas depending on BT: 0.51 to 0.75 mm (3.8%), 0.76 to 0.90 mm (5.3%), and 0.91 to 1.00 mm (10.3%) [34]. Patients with BT < 0.5 mm in the study by Murali et al. did not have any positive SLNB ( ) [34]. In our study, SLNB was performed on 61.3% (65/106) of the thin melanomas examined and was positive in 4.6% (3/65) of patients. If we applied BT < 0.50 mm as a cutoff point as in the study by Murali et al., in our cohort patients with thin melanomas with BT < 0.50 mm had no positive SLNB (0/15). In contrast, patients with thin melanomas of BT ≥ 0.50 mm had a 6.0% (3/50) rate of positive SLNB.

All patients in this study with SLN micrometastasis lacked regression or an associated nevus. This is consistent with contemporary studies demonstrating that regression is not a predictor of a positive SLNB in thin melanomas [3537]. Previously, the presence of regression was considered to be a risk factor for SLN metastasis; however, based on currently available data, standard clinical practice does not generally incorporate regression as a criterion for SLNB in patients with thin melanomas [8, 3739].

However, it should be noted that a study by Guitart et al. demonstrated that a subset of thin melanomas with extensive regression (defined as regression in 50% or more of the lesion) poses a risk for metastasis [40]. Further studies are needed to determine if the extent of regression (defined as a percentage of the lesion) correlates with prognosis or SLN status.

5. Conclusion

This study corroborates the importance of MR in thin melanomas as a risk factor for SLN metastasis and the role of SLNB in a subset of patients with thin melanomas [16, 22, 34, 41, 42]. All patients with positive SLNB were classified as T1b according to 2001 and 2009 staging systems. While it is evident that a subset of thin melanomas behaves aggressively with some risk for distant metastasis and death from melanoma, the clinical-pathologic parameters for SLNB in patients with thin melanomas continue to evolve [7, 14, 43]. In particular, CL IV invasion in thin melanomas has been described by some authors to pose a risk for SLN metastasis [41, 4447], while other studies have demonstrated CL IV invasion not to be predictive of SLN involvement in tumors when compared to BT and ulceration [48, 49]. Previous studies have shown by univariate analysis clinical-pathologic factors such as BT, CL, MR, ulceration status, anatomic site, age, and gender as significant predictors of nodal metastasis in thin melanomas [47, 50]. However, by multivariate analysis, only BT, gender, and age remained significant for thin lesions [43, 47]. Factors to be considered for SLNB in patients with thin melanomas among at least some melanoma experts include patients with BT ≥ 0.76, MR ≥ 1/mm2, primary tumor ulceration, “young” age, and superficially shaved lesions with positive deep margins which may underestimate the actual BT [28, 31, 34, 44, 47, 49, 51]. However, ultimately the selection of a subset of patients with thin melanomas for SLNB requires coordinated discussion between the patient and clinician.

This is a retrospective study and is limited by a small sample size ( ) of patients with thin primary melanomas; of note, only 3 patients in this cohort had a positive SLNB. Furthermore, a selection bias towards more aggressive disease may be introduced in our patient sample since our institution is a referral center for high-risk patients. To further address significant clinical-pathological features that define a subset of patients with higher risk thin melanomas we intend to examine a larger cohort of thin cutaneous melanomas.

Continued reporting of all the histologic parameters as previously described for all (including thin) melanomas will permit incorporation of all primary tumor characteristics into clinical management and facilitate continued collection of data to further assess “high-risk” clinical-pathologic factors in patients with thin melanomas [52].

Disclosure

Portion of these data was included in a poster presentation entitled “Implications of the 2009 AJCC Melanoma Staging and Classification System for Thin Melanomas” at the United States and Canadian Academy of Pathology Annual Meeting, March 17–23, 2012, Vancouver, BC, Canada. Reprint request: Jonathan L. Curry, MD, Department of Pathology, MDACC, Houston, USA.

Conflict of Interests

Dr. Gershenwald has been consultant for Navidea Corporation. The remaining authors have no conflict of interests to declare.

References

  1. D. S. Rigel, “Epidemiology of melanoma,” Seminars in Cutaneous Medicine and Surgery, vol. 29, no. 4, pp. 204–209, 2010. View at Publisher · View at Google Scholar · View at Scopus
  2. A. Jemal, R. Siegel, E. Ward, Y. Hao, J. Xu, and M. J. Thun, “Cancer statistics, 2009,” CA: Cancer Journal for Clinicians, vol. 59, no. 4, pp. 225–249, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. J. E. Frangos, L. M. Duncan, A. Piris et al., “Increased diagnosis of thin superficial spreading melanomas: a 20-year study,” Journal of the American Academy of Dermatology, vol. 67, no. 3, pp. 387–394, 2012. View at Publisher · View at Google Scholar · View at Scopus
  4. A. Jemal, M. Saraiya, P. Patel et al., “Recent trends in cutaneous melanoma incidence and death rates in the United States, 1992–2006,” Journal of the American Academy of Dermatology, vol. 65, no. 5, supplement 1, pp. S17.e1–S17.e11, 2011. View at Publisher · View at Google Scholar · View at Scopus
  5. A. Breslow, “Thickness, cross-sectional areas and depth of invasion in the prognosis of cutaneous melanoma,” Annals of Surgery, vol. 172, no. 5, pp. 902–908, 1970. View at Scopus
  6. C. M. Balch, A. C. Buzaid, S. J. Soong et al., “Final version of the American joint committee on cancer staging system for cutaneous melanoma,” Journal of Clinical Oncology, vol. 19, no. 16, pp. 3635–3648, 2001. View at Scopus
  7. C. M. Balch, J. E. Gershenwald, S. Soong et al., “Final version of 2009 AJCC melanoma staging and classification,” Journal of Clinical Oncology, vol. 27, no. 36, pp. 6199–6206, 2009. View at Publisher · View at Google Scholar · View at Scopus
  8. W. H. Clark Jr., D. E. Elder, D. T. Guerry et al., “Model predicting survival in stage I melanoma based on tumor progression,” Journal of the National Cancer Institute, vol. 81, no. 24, pp. 1893–1904, 1989. View at Scopus
  9. W. H. Clark Jr., L. From, E. A. Bernardino, and M. C. Mihm, “The histogenesis and biologic behavior of primary human malignant melanomas of the skin,” Cancer Research, vol. 29, no. 3, pp. 705–727, 1969. View at Scopus
  10. A. Breslow, “Tumor thickness, level of invasion and node dissection in stage I cutaneous melanoma,” Annals of Surgery, vol. 182, no. 5, pp. 572–575, 1975. View at Scopus
  11. A. C. Halpern and A. A. Marghoob, “Thin melanoma: still “excellent prognosis” disease?” Journal of Clinical Oncology, vol. 22, no. 18, pp. 3651–3653, 2004. View at Publisher · View at Google Scholar · View at Scopus
  12. D. E. Elder, “Thin melanoma,” Archives of Pathology and Laboratory Medicine, vol. 135, no. 3, pp. 342–346, 2011.
  13. J. F. Thompson, S. Soong, C. M. Balch et al., “Prognostic significance of mitotic rate in localized primary cutaneous melanoma: an analysis of patients in the multi-institutional American joint committee on cancer melanoma staging database,” Journal of Clinical Oncology, vol. 29, no. 16, pp. 2199–2205, 2011. View at Publisher · View at Google Scholar · View at Scopus
  14. C. M. Balch, J. E. Gershenwald, S. Soong, and J. F. Thompson, “Update on the melanoma staging system: the importance of sentinel node staging and primary tumor mitotic rate,” Journal of Surgical Oncology, vol. 104, no. 4, pp. 379–385, 2011. View at Publisher · View at Google Scholar · View at Scopus
  15. M. F. Azzola, H. M. Shaw, J. F. Thompson et al., “Tumor mitotic rate is a more powerful prognostic indicator than ulceration in patients with primary cutaneous melanoma: an analysis of 3661 patients from a single center,” Cancer, vol. 97, no. 6, pp. 1488–1498, 2003. View at Publisher · View at Google Scholar · View at Scopus
  16. A. B. Francken, H. M. Shaw, J. F. Thompson et al., “The prognostic importance of tumor mitotic rate confirmed in 1317 patients with primary cutaneous melanoma and long follow-up,” Annals of Surgical Oncology, vol. 11, no. 4, pp. 426–433, 2004. View at Publisher · View at Google Scholar · View at Scopus
  17. P. A. Gimotty and D. Guerry, “Prognostication in thin cutaneous melanomas,” Archives of Pathology and Laboratory Medicine, vol. 134, no. 12, pp. 1758–1763, 2010. View at Scopus
  18. J. E. Gershenwald, W. Thompson, P. F. Mansfield et al., “Multi-institutional melanoma lymphatic mapping experience: the prognostic value of sentinel lymph node status in 612 stage I or II melanoma patients,” Journal of Clinical Oncology, vol. 17, no. 3, pp. 976–983, 1999. View at Scopus
  19. G. M. Boland and J. E. Gershenwald, “Sentinel lymph node biopsy in melanoma,” Cancer Journal, vol. 18, no. 2, pp. 185–191, 2012. View at Publisher · View at Google Scholar · View at Scopus
  20. J. E. Gershenwald and M. I. Ross, “Sentinel-lymph-node biopsy for cutaneous melanoma,” The New England Journal of Medicine, vol. 364, no. 18, pp. 1738–1745, 2011. View at Publisher · View at Google Scholar · View at Scopus
  21. D. L. Morton, D. R. Wen, J. H. Wong et al., “Technical details of intraoperative lymphatic mapping for early stage melanoma,” Archives of Surgery, vol. 127, no. 4, pp. 392–399, 1992. View at Scopus
  22. R. H. I. Andtbacka and J. E. Gershenwald, “Role of sentinel lymph node biopsy in patients with thin melanoma,” Journal of the National Comprehensive Cancer Network, vol. 7, no. 3, pp. 308–317, 2009. View at Scopus
  23. C. Schmoeckel and O. Braun-Falco, “Prognostic index in malignant melanoma,” Archives of Dermatology, vol. 114, no. 6, pp. 871–873, 1978. View at Publisher · View at Google Scholar · View at Scopus
  24. V. J. McGovern, A. J. Cochran, and E. P. van der Esch, “The classification of malignant melanoma, its histological reporting and registration: a revision of the 1972 Sydney classification,” Pathology, vol. 18, no. 1, pp. 12–21, 1986. View at Scopus
  25. V. G. Prieto, “Sentinel lymph nodes in cutaneous melanoma: handling, examination, and clinical repercussion,” Archives of Pathology and Laboratory Medicine, vol. 134, no. 12, pp. 1764–1769, 2010. View at Scopus
  26. V. G. Prieto and S. H. Clark, “Processing of sentinel lymph nodes for detection of metastatic melanoma,” Annals of Diagnostic Pathology, vol. 6, no. 4, pp. 257–264, 2002. View at Publisher · View at Google Scholar · View at Scopus
  27. V. G. Prieto, Sentinel Lymph Node Biopsy in Melanoma Pathology, edited by J. A. Reed, Medscape, 2011.
  28. S. A. Sekula-Gibbs and M. A. Shearer, “Sentinel node biopsy should be offered in thin melanoma with mitotic rate greater than one,” Dermatologic Surgery, vol. 37, no. 8, pp. 1080–1088, 2011. View at Publisher · View at Google Scholar · View at Scopus
  29. A. Piris, M. C. Mihm Jr., and L. M. Duncan, “AJCC melanoma staging update: impact on dermatopathology practice and patient management,” Journal of Cutaneous Pathology, vol. 38, no. 5, pp. 394–400, 2011. View at Publisher · View at Google Scholar · View at Scopus
  30. P. V. Dickson and J. E. Gershenwald, “Staging and prognosis of cutaneous melanoma,” Surgical Oncology Clinics of North America, vol. 20, no. 1, pp. 1–17, 2011. View at Publisher · View at Google Scholar · View at Scopus
  31. J. E. Gershenwald, D. G. Coit, V. K. Sondak, and J. F. Thompson, “The challenge of defining guidelines for sentinel lymph node biopsy in patients with thin primary cutaneous melanomas,” Annals of Surgical Oncology, vol. 19, no. 11, pp. 3301–3303, 2012. View at Publisher · View at Google Scholar
  32. M. I. Ross and J. E. Gershenwald, “Evidence-based treatment of early-stage melanoma,” Journal of Surgical Oncology, vol. 104, no. 4, pp. 341–353, 2011. View at Publisher · View at Google Scholar · View at Scopus
  33. D. V. Yonick, R. M. Ballo, E. Kahn et al., “Predictors of positive sentinel lymph node in thin melanoma,” The American Journal of Surgery, vol. 201, no. 3, pp. 324–328, 2011. View at Publisher · View at Google Scholar · View at Scopus
  34. R. Murali, L. E. Haydu, M. J. Quinn et al., “Sentinel lymph node biopsy in patients with thin primary cutaneous melanoma,” Annals of Surgery, vol. 255, no. 1, pp. 128–133, 2012. View at Publisher · View at Google Scholar · View at Scopus
  35. A. Testori, G. L. de Salvo, M. C. Montesco et al., “Clinical considerations on sentinel node biopsy in melanoma from an Italian multicentric study on 1,313 patients (SOLISM-IMI),” Annals of Surgical Oncology, vol. 16, no. 7, pp. 2018–2027, 2009. View at Publisher · View at Google Scholar · View at Scopus
  36. K. T. Morris, K. J. Busam, S. Bero, A. Patel, and M. S. Brady, “Primary cutaneous melanoma with regression does not require a lower threshold for sentinel lymph node biopsy,” Annals of Surgical Oncology, vol. 15, no. 1, pp. 316–322, 2008. View at Publisher · View at Google Scholar · View at Scopus
  37. S. E. McClain, A. L. Shada, M. Barry, J. W. Patterson, and C. L. Slingluff Jr., “Outcome of sentinel lymph node biopsy and prognostic implications of regression in thin malignant melanoma,” Melanoma Research, vol. 22, no. 4, pp. 302–309, 2012. View at Publisher · View at Google Scholar
  38. A. L. Burton, J. Gilbert, R. W. Farmer et al., “Regression does not predict nodal metastasis or survival in patients with cutaneous melanoma,” The American Surgeon, vol. 77, no. 8, pp. 1009–1013, 2011. View at Scopus
  39. J. Oláh, R. Gyulai, I. Korom, E. Varga, and A. Dobozy, “Tumour regression predicts higher risk of sentinel node involvement in thin cutaneous melanomas,” The British Journal of Dermatology, vol. 149, no. 3, pp. 662–663, 2003. View at Publisher · View at Google Scholar · View at Scopus
  40. J. Guitart, L. Lowe, M. Piepkorn et al., “Histological characteristics of metastasizing thin melanomas: a case-control study of 43 cases,” Archives of Dermatology, vol. 138, no. 5, pp. 603–608, 2002. View at Scopus
  41. B. E. Wright, R. P. Scheri, X. Ye et al., “Importance of sentinel lymph node biopsy in patients with thin melanoma,” Archives of Surgery, vol. 143, no. 9, pp. 892–899, 2008. View at Publisher · View at Google Scholar · View at Scopus
  42. M. A. Nading, C. M. Balch, and A. J. Sober, “Implications of the 2009 American joint committee on cancer melanoma staging and classification on dermatologists and their patients,” Seminars in Cutaneous Medicine and Surgery, vol. 29, no. 3, pp. 142–147, 2010. View at Publisher · View at Google Scholar · View at Scopus
  43. R. Murali, L. E. Haydu, G. V. Long et al., “Clinical and pathologic factors associated with distant metastasis and survival in patients with thin primary cutaneous melanoma,” Annals of Surgical Oncology, vol. 19, no. 6, pp. 1782–1789, 2012. View at Publisher · View at Google Scholar · View at Scopus
  44. V. P. Koshenkov, D. Shulkin, R. Bustami, A. H. Chevinsky, and E. D. Whitman, “Role of sentinel lymphadenectomy in thin cutaneous melanomas with positive deep margins on initial biopsy,” Journal of Surgical Oncology, vol. 106, no. 4, pp. 363–368, 2012. View at Publisher · View at Google Scholar · View at Scopus
  45. J. G. McKinnon, X. Q. Yu, W. H. McCarthy, and J. F. Thompson, “Prognosis for patients with thin cutaneous melanoma: long-term survival data from the New South Wales central cancer registry and the Sydney melanoma unit,” Cancer, vol. 98, no. 6, pp. 1223–1231, 2003. View at Publisher · View at Google Scholar · View at Scopus
  46. J. M. Ranieri, J. D. Wagner, S. Wenck, C. S. Johnson, and J. J. Coleman III, “The prognostic importance of sentinel lymph node biopsy in thin melanoma,” Annals of Surgical Oncology, vol. 13, no. 7, pp. 927–932, 2006. View at Publisher · View at Google Scholar · View at Scopus
  47. M. B. Faries, L. A. Wanek, D. Elashoff, B. E. Wright, and D. L. Morton, “Predictors of occult nodal metastasis in patients with thin melanoma,” Archives of Surgery, vol. 145, no. 2, pp. 137–142, 2010. View at Publisher · View at Google Scholar · View at Scopus
  48. C. A. Puleo, J. L. Messina, A. I. Riker et al., “Sentinel node biopsy for thin melanomas: which patients should be considered?” Cancer Control, vol. 12, no. 4, pp. 230–235, 2005. View at Scopus
  49. D. Han, D. Yu, X. Zhao, et al., “Sentinel node biopsy is indicated for thin melanomas ≥0.76 mm,” Annals of Surgical Oncology, vol. 19, no. 11, pp. 3335–3342, 2012. View at Publisher · View at Google Scholar
  50. A. S. Caudle, M. I. Ross, V. G. Prieto et al., “Mitotic rate predicts sentinel lymph node involvement in melanoma: impact of the 7th edition AJCC melanoma staging system,” Annals of Surgical Oncology, vol. 17, no. 8, 2010.
  51. R. Murali, R. A. Scolyer, and J. F. Thompson, “Can we better identify thin cutaneous melanomas that are likely to metastasize and cause death?” Annals of Surgical Oncology, vol. 19, no. 11, pp. 3310–3312, 2012. View at Publisher · View at Google Scholar
  52. D. Ivan and V. G. Prieto, “An update on reporting histopathologic prognostic factors in melanoma,” Archives of Pathology and Laboratory Medicine, vol. 135, no. 7, pp. 825–829, 2011. View at Scopus