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BioMed Research International
Volume 2013 (2013), Article ID 901821, 14 pages
http://dx.doi.org/10.1155/2013/901821
Research Article

Isolation, Characterization, and Transduction of Endometrial Decidual Tissue Multipotent Mesenchymal Stromal/Stem Cells from Menstrual Blood

1Division of Oncology, Department of Medical and Surgical Sciences of Children & Adults, University Hospital of Modena and Reggio Emilia, Via del Pozzo 71, 41100 Modena, Italy
2Department of Internal Medicine and Oncology, University of Bari Aldo Moro, Bari, Italy
3Unit of Plastic Surgery, Department of Medical and Surgical Sciences of Children & Adults, University Hospital of Modena and Reggio Emilia, Via del Pozzo 71, 41100 Modena, Italy
4Division of Pediatric Oncology, Hematology and Marrow Transplantation, Department of Medical and Surgical Sciences of Children & Adults, University Hospital of Modena and Reggio Emilia, Via del Pozzo 71, 41100 Modena, Italy
5Division of Oncology, Children’s Hospital of Philadelphia, Philadelphia, PA 19104, USA

Received 2 November 2012; Revised 22 January 2013; Accepted 28 January 2013

Academic Editor: Thomas Skutella

Copyright © 2013 Filippo Rossignoli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mesenchymal stromal/stem cells (MSCs) reveal progenitor cells-like features including proliferation and differentiation capacities. One of the most historically recognized sources of MSC has been the bone marrow, while other sources recently include adipose tissue, teeth, bone, muscle, placenta, liver, pancreas, umbilical cord, and cord blood. Frequently, progenitor isolation requires traumatic procedures that are poorly feasible and associated with patient discomfort. In the attempt to identify a more approachable MSC source, we focused on endometrial decidual tissue (EDT) found within menstrual blood. Based also on recent literature findings, we hypothesized that EDT may contain heterogeneous populations including some having MSC-like features. Thus, we here sought to isolate EDT-MSC processing menstrual samples from multiple donors. Cytofluorimetric analyses revealed that resulting adherent cells were expressing mesenchymal surface markers, including CD56, CD73, CD90, CD105 and CD146, and pluripotency markers such as SSEA-4. Moreover, EDT-MSC showed a robust clonogenic potential and could be largely expanded in vitro as fibroblastoid elements. In addition, differentiation assays drove these cells towards osteogenic, adipogenic, and chondrogenic lineages. Finally, for the first time, we were able to gene modify these progenitors by a retroviral vector carrying the green fluorescent protein. From these data, we suggest that EDT-MSC could represent a new promising tool having potential within cell and gene therapy applications.