901821.fig.004a
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901821.fig.004b
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901821.fig.004c
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901821.fig.004d
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Figure 4: β-galactosidase staining. (a) Activity of β-galactosidase at pH 6 was assessed on passage 3 EDT-MSC cultured in FBS resulting almost negative for the presence of typical blue senescent cells. The conspicuous number of mitotic event is also visible, highlighting an intense proliferative activity. (b) Cells grown in Quantum 333 underwent senescence at passage 17 and resulted in deep blue staining within most of the amplified EDT-MSC. (c) Cells cultured in FBS were capable to reach passage 26 before showing senescence features, such as blue staining, large cytoplasm, multinucleated elements, and evident cytoskeleton. (d) A detail of a senescent cell. Original magnification 100x.