Table 1: Primer sequences.

GenePrimer sequenceAmplified length

β-actin5′-ACC TTC TAC AAT GAG CTG CG-3′ (sense)148 bp
5′-CCT GGA TAG CAA CGT ACA TGG-3′ (antisense)
ALP5′-GAT GTG GAG TAT GAG AGT GAC G-3′ (sense)142 bp
5′-GGT CAA GGG TCA GGA GTT C-3′ (antisense)
COL1A25′-AGG ACA AGA AAC ACG TCT GG-3′ (sense)146 bp
5′-GGT GAT GTT CTG AGA GGC ATA G-3′ (antisense)
OC5′-CAG CGA GGT AGT GAA GAG AC-3′ (sense)144 bp
5′-TGA AAG CCG ATG TGG TCA G-3′ (antisense)

ALP: alkaline phosphatase, COL1A2: collagen 1A2, OC: osteocalcin.