Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-Like/Gyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom
Figure 1
(a) Elution profile of L. muta rhombeata venom by the reversed phase HPLC on C8 Discovery BIO Wide Pore. Fraction 16 (F16) that contained both amidolytic and thrombin-like activities (Rhombeobin) from L. muta rhombeata venom is indicated. (b) Elution profile of F8 following RP-HPLC on C8 Discovery BIO Wide Pore. Insert: SDS-PAGE in nonreduced (NR) and reduced (R) conditions. (c) Molecular mass determination of the native Rhombeobin by nanoelectrospray tandem mass spectrometry, using a Q-Tof Ultima API mass spectrometer (MicroMass/Waters) with output mass range of 40.000–50.000 Da at a “resolution” of 0.1 Da/channel.
