Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-Like/Gyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom
Figure 6
(a) Relative levels of mouse plasma fibrinogen after intravenous injection of PBS (■) or Rhombeobin: 0.1 μg (●) and 1 μg (▲). Values were expressed as % compared with the control. Rhombeobin fibrinogenolytic activity at different incubation times: (1) bovine fibrinogen; (2) fibrinogen + Rhombeobin (5 min); (3) 15 min; (4) 30 min; (5) 1 h; (6) 2 h; 0.1 μg (b) or 1 μg (c) of enzyme mixture with bovine fibrinogen solution (2 mg/mL) and analysis by SDS-PAGE (12.5%); MW: molecular markers. Error bars indicate mean plus or minus SME (); * means significant difference () compared with the control.
