Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-Like/Gyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom

Table 2

Measured molecular masses and deduced amino acid sequences obtained by ESI-Q-Tof-MS/MS based on the alkylated SV-8 peptides of Rhombeobin.

Peptide	Position	Amino acid sequence	Measured mass (Da)
S-1	1–11	VI/LGGDECNINE	1218.5202
S-2	12–36	HRFI/LVAI/LYDGI/LSGTFI/LCGGTI/LI/LNQE	2780.3842
S-3	37–48	WVI/LTAAHCDSE	1287.5534
S-4	66–72	QTRYPKE	920.4763
S-5	73–82	KYFFRCPNKKNDE	1744.8213
S-6	134–150	TI/LPDVPHCANI/LNI/LI/LDYE	1982.8704
S-7	151–174	VCRAAYAGI/LPATSRVI/LCAGVI/LE	2333.1693

The peptides were separated by RP-HPLC and were sequenced by mass spectrometry. C = alkylated cysteine; glutamic acid residues shown in bold were deduced on the cleavage Streptoccocus aureus SV-8. All molecular masses are reported as monoisotopic.