Preliminary Characterization of Genipin-Cross-Linked Silk Sericin/Poly(vinyl alcohol) Films as Two-Dimensional Wound Dressings for the Healing of Superficial Wounds

Table 3

Average intensity of inflammatory cells, necrosis, fibrosis, neovascularization, and fatty infiltrate in non-cross-linked silk sericin/PVA films, 0.1% w/v genipin-cross-linked silk sericin/PVA films, and Sofra tulle after subcutaneous implantation for 3, 7, 14, and 28 days.

	3 d			7 d			14 d			28 d
	A*	B**	C***	A	B	C	A	B	C	A	B	C
PMN	4^†	4	3	2.8	3.3	0.8	0.8	3	0	0	3.8	0
Lymphocytes	2.8	2.8	1.5	3	3	2	3.5	3	2.3	3	3	1
Plasma cells	0	0	0	0	0	0	0	0	0	0	0.8	0
Macrophages	2.3	2.8	3	3	3	2	3.3	3	3	3	3	2.3
Giant cells	0	0	0	0	0	2.3	3.3	1.3	3.8	2.3	1	4
Necrosis	1	0	0	1	0	0	1.5	1.5	0	1.5	0	0
Fibrosis	1.8	0.8	1.8	3.3	4	2.8	3.5	4	3	3	3.5	2.5
Neovascularization	3	3	3	3	2.8	2.3	3	3	2.8	1	3	1.3
Fatty infiltrate	0	0	0	0	1	2.3	0	0	2.3	0.5	0	3

 *A: non-cross-linked silk sericin/PVA films.
**B: 0.1% w/v genipin-cross-linked silk sericin/PVA films.
***C: Sofra tulle (control).
^†Intensity 0–4: 0 = not observed, 1 = rare, 2 = minimal, 3 = heavily infiltrate, and 4 = packed infiltrate.