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BioMed Research International
Volume 2013 (2013), Article ID 905252, 10 pages
Cytogenetic Abnormality in Exfoliated Cells of Buccal Mucosa in Head and Neck Cancer Patients in the Tunisian Population: Impact of Different Exposure Sources
1Unit of Marine and Environmental Toxicology, UR 09-03, Sfax University, IPEIS, BP 1172, 3018 Sfax, Tunisia
2Unit of Bioinformatics and Human Genetics, Centre of Biotechnology of Sfax, BP 1177, 3018 Sfax, Tunisia
3Department of Otorhinolaryngology, Habib Bourguiba Hospital, 3000 Sfax, Tunisia
Received 6 April 2013; Accepted 11 June 2013
Academic Editor: Manoor Prakash Hande
Copyright © 2013 Rim Khlifi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- B. Rodu and C. Jansson, “Smokeless tobacco and oral cancer: a review of the risks and determinants,” Critical Reviews in Oral Biology and Medicine, vol. 15, no. 5, pp. 252–263, 2004.
- K. Jensen, A. B. Jensen, and C. Grau, “Smoking has a negative impact upon health related quality of life after treatment for head and neck cancer,” Oral Oncology, vol. 43, no. 2, pp. 187–192, 2007.
- C. Daneş, “Head and neck occupational cancer ethypathologenic aspects,” Acta Madica Transilvanica, vol. 21, pp. 266–268, 2012.
- R. Khlifi, P. Olmedo, F. Gil et al., “Blood nickel and chromium levels in association with smoking and occupational exposure among head and neck cancer patients in Tunisia,” Environmental Science and Pollution Research, 2013.
- U. A. Harréus, N. H. Kleinsasser, S. Zieger et al., “Sensitivity to DNA-damage induction and chromosomal alterations in mucosa cells from patients with and without cancer of the oropharynx detected by a combination of Comet assay and fluorescence in situ hybridization,” Mutation Research, vol. 563, no. 2, pp. 131–138, 2004.
- N. H. Kleinsasser, B. C. Wallner, C. Wagner, E. R. Kastenbauer, and U. A. Harréus, “DNA repair capacity in lymphocytes of nasopharyngeal cancer patients,” European Archives of Oto-Rhino-Laryngology, vol. 262, no. 7, pp. 561–566, 2005.
- S. D. Spivack, G. J. Hurteau, R. Jain et al., “Gene-environment interaction signatures by quantitative mRNA profiling in exfoliated buccal mucosal cells,” Cancer Research, vol. 64, no. 18, pp. 6805–6813, 2004.
- F. D. Martinez, “Gene-environment interactions in asthma and allergies: a new paradigm to understand disease causation,” Immunology and Allergy Clinics of North America, vol. 25, no. 4, pp. 709–721, 2005.
- H. E. Gabriel, J. W. Crott, H. Ghandour et al., “Chronic cigarette smoking is associated with diminished folate status, altered folate form distribution, and increased genetic damage in the buccal mucosa of healthy adults,” American Journal of Clinical Nutrition, vol. 83, no. 4, pp. 835–841, 2006.
- C. Chen, M. Arjomandi, H. Qin, J. Balmes, I. Tager, and N. Holland, “Cytogenetic damage in buccal epithelia and peripheral lymphocytes of young healthy individuals exposed to ozone,” Mutagenesis, vol. 21, no. 2, pp. 131–137, 2006.
- G. Speit and O. Schmid, “Local genotoxic effects of formaldehyde in humans measured by the micronucleus test with exfoliated epithelial cells,” Mutation Research, vol. 613, no. 1, pp. 1–9, 2006.
- N. K. Proia, G. M. Paszkiewicz, M. A. S. Nasca, G. E. Franke, and J. L. Pauly, “Smoking and smokeless tobacco-associated human buccal cell mutations and their association with oral cancer—a review,” Cancer Epidemiology Biomarkers and Prevention, vol. 15, no. 6, pp. 1061–1077, 2006.
- N. Holland, C. Bolognesi, M. Kirsch-Volders et al., “The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps,” Mutation Research, vol. 659, no. 1-2, pp. 93–108, 2008.
- H. F. Stich and M. P. Rosin, “Quantitating the synergistic effect of smoking and alcohol consumption with the micronucleus test on human buccal mucosa cells,” International Journal of Cancer, vol. 31, no. 3, pp. 305–308, 1983.
- W. W. Au, “Cytogenetic assays in monitoring human exposure and prediction of risk,” Environmental Mutagens, Carcinogens, and Teratogens, vol. 23, pp. 236–245, 1991.
- M. Fenech, N. Holland, W. P. Chang, E. Zeiger, and S. Bonassi, “The HUman MicroNucleus Project—an international collaborative study on the use of the micronucleus technique for measuring DNA damage in humans,” Mutation Research, vol. 428, no. 1-2, pp. 271–283, 1999.
- M. Fenech, “The in vitro micronucleus technique,” Mutation Research, vol. 455, no. 1-2, pp. 81–95, 2000.
- M. Fenech, P. Baghurst, W. Luderer et al., “Low intake of calcium, folate, nicotinic acid, vitamin E, retinol, β-carotene and high intake of pantothenic acid, biotin and riboflavin are significantly associated with increased genome instability—results from a dietary intake and micronucleus index survey in South Australia,” Carcinogenesis, vol. 26, no. 5, pp. 991–999, 2005.
- K. Umegaki and M. Fenech, “Cytokinesis-block micronucleus assay in WIL2-NS cells: a sensitive system to detect chromosomal damage induced by reactive oxygen species and activated human neutrophils,” Mutagenesis, vol. 15, no. 3, pp. 261–269, 2000.
- R. Mateuca, N. Lombaert, P. V. Aka, I. Decordier, and M. Kirsch-Volders, “Chromosomal changes: induction, detection methods and applicability in human biomonitoring,” Biochimie, vol. 88, no. 11, pp. 1515–1531, 2006.
- M. Fenech, W. P. Chang, M. Kirsch-Volders, N. Holland, S. Bonassi, and E. Zeiger, “HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures,” Mutation Research, vol. 534, no. 1-2, pp. 65–75, 2003.
- M. G. Martino-Roth, J. Viégas, M. Amaral, L. Oliveira, F. L. S. Ferreira, and B. Erdtmann, “Evaluation of genotoxicity through micronuclei test in workers of car and battery repair garages,” Genetics and Molecular Biology, vol. 25, no. 4, pp. 495–500, 2002.
- S. B. A. Ayyad, E. Israel, M. El-Setouhy, G. R. Nasr, M. K. Mohamed, and C. A. Loffredo, “Evaluation of papanicolaou stain for studying micronuclei in buccal cells under field conditions,” Acta Cytologica, vol. 50, no. 4, pp. 398–402, 2006.
- P. E. Tolbert, C. M. Shy, and J. W. Allen, “Micronuclei and other nuclear anomalies in buccal smears: methods development,” Mutation Research, vol. 271, no. 1, pp. 69–77, 1992.
- N. Titenko-Holland, R. A. Jacob, N. Shang, A. Balaraman, and M. T. Smith, “Micronuclei in lymphocytes and exfoliated buccal cells of postmenopausal women with dietary changes in folate,” Mutation Research, vol. 417, no. 2-3, pp. 101–114, 1998.
- D. M. Roberts, “Comparative cytology of the oral cavities of snuff users,” Acta Cytologica, vol. 41, no. 4, pp. 1008–1014, 1997.
- M. Fenech, “Chromosomal biomarkers of genomic instability relevant to cancer,” Drug Discovery Today, vol. 7, no. 22, pp. 1128–1137, 2002.
- M. Bloching, A. Hofman, A. Berghaus, C. Lautenschläger, and T. Grummt, “Micronuclei as biological markers for the detection of local cancer transformation in the upper aerodigestive tract,” HNO, vol. 48, pp. 444–450, 2000.
- F. J. Aceves Avila, G. A. Esquivel Nava, M. P. Gallegos Arreola, B. C. Gómez Meda, G. M. Zúñiga González, and C. Ramos-Remus, “Cyclophosphamide boluses induce micronuclei expression in buccal mucosa cells of patients with systemic lupus erythematosus independent of cytochrome P450 2D6 status,” Journal of Rheumatology, vol. 31, no. 7, pp. 1335–1339, 2004.
- C. Ramos-Remus, G. Dorazco-Barragan, F. J. Aceves-Avila et al., “Genotoxicity assessment using micronuclei assay in rheumatoid arthritis patients,” Clinical and Experimental Rheumatology, vol. 20, no. 2, pp. 208–212, 2002.
- S. R. D. A. Reis, M. Sadigursky, M. G. S. Andrade, L. P. Soares, A. R. D. Espirito Santo, and D. S. Vilas Boas, “Genotoxic effect of ethanol on oral mucosa cells,” Brazilian Oral Research, vol. 16, no. 3, pp. 221–225, 2002.
- L. E. Moore, M. L. Warner, A. H. Smith, et al., “Use of fluorescence micronucleus assay to detect the genotoxic effects of radiation and arsenic exposure in exfoliated human epithelial cells,” Environmental and Molecular Mutagenesis, vol. 27, no. 3, pp. 176–184, 1996.
- M. P. R. Prasad, M. A. Mukundan, and K. Krishnaswamy, “Micronuclei and carcinogen DNA adducts as intermediate end points in nutrient intervention trial of precancerous lesions in the oral cavity,” European Journal of Cancer B, vol. 31, no. 3, pp. 155–159, 1995.
- R. Saran, R. K. Tiwari, P. P. Reddy, and Y. R. Ahuja, “Risk assessment of oral cancer in patients with pre-cancerous states of the oral cavity using micronucleus test and challenge assay,” Oral Oncology, vol. 44, no. 4, pp. 354–360, 2008.
- S. Burgaz, E. Coskun, G. C. Demircigil et al., “Micronucleus frequencies in lymphocytes and buccal epithelial cells from patients having head and neck cancer and their first-degree relatives,” Mutagenesis, vol. 26, no. 2, pp. 351–356, 2011.
- J. Cao, H. W. Liu, X. S. Liu, J. Q. Jin, and P. Zhang, “Correlation between the quantity of oral mucosal micronucleus cells and cancerization,” Beijing Da Xue Xue Bao, vol. 43, no. 4, pp. 600–602, 2011.
- A. C. Pellicioli, F. Visioli, L. A. Ferreira, et al., “Cytogenetic abnormalities in exfoliated oral mucosal cells and their association with oral cancer,” Analytical and Quantitative Cytology and Histology, vol. 33, no. 5, pp. 271–276, 2011.
- L. T. Dórea, J. R. Meireles, J. P. Lessa, et al., “Chromosomal damage and apoptosis in exfoliated buccal cells from individuals with oral cancer,” International Journal of Dentistry, vol. 2012, Article ID 458054, 2012.
- F. Duffaud, T. Orsière, L. Digue et al., “Micronucleated lymphocyte rates from head-and-neck cancer patients,” Mutation Research, vol. 439, no. 2, pp. 259–266, 1999.
- E. M. Minicucci, L. P. Kowalski, M. A. C. Maia et al., “Cytogenetic damage in circulating lymphocytes and buccal mucosa cells of head-and-neck cancer patients undergoing radiotherapy,” Journal of Radiation Research, vol. 46, no. 2, pp. 135–142, 2005.
- H. Hintzsche, B. Polat, V. Schewe, et al., “Micronucleus formation kinetics in buccal mucosa cells of head and neck cancer patients undergoing radiotherapy,” Toxicology Letters, vol. 212, no. 1, pp. 33–37, 2012.
- M. E. Gonsebatt, L. Vega, A. M. Salazar et al., “Cytogenetic effects in human exposure to arsenic,” Mutation Research, vol. 386, no. 3, pp. 219–228, 1997.
- S. Pastor, S. Gutiérrez, A. Creus, N. Xamena, S. Piperakis, and R. Marcos, “Cytogenetic analysis of Greek farmers using the micronucleus assay in peripheral lymphocytes and buccal cells,” Mutagenesis, vol. 16, no. 6, pp. 539–545, 2001.
- Y. Ozkul, H. Donmez, A. Erenmemisoglu, H. Demirtas, and N. Imamoglu, “Induction of micronuclei by smokeless tobacco on buccal mucosa cells of habitual users,” Mutagenesis, vol. 12, no. 4, pp. 285–287, 1997.
- G. J. F. Gattás, L. de Almeida Cardoso, M. de Araújo Medrado-Faria, and P. H. Saldanha, “Frequency of oral mucosa micronuclei in gas station operators after introducing methanol,” Occupational Medicine, vol. 51, no. 2, pp. 107–113, 2001.
- D. Pinto, J. M. Ceballos, G. García et al., “Increased cytogenetic damage in outdoor painters,” Mutation Research, vol. 467, no. 2, pp. 105–111, 2000.
- M. Konopacka, “Effect of smoking and aging on micronucleus frequencies in human exfoliated buccal cells,” Neoplasma, vol. 50, no. 5, pp. 380–382, 2003.
- M. Fenech and S. Bonassi, “The effect of age, gender, diet and lifestyle on DNA damage measured using micronucleus frequency in human peripheral blood lymphocytes,” Mutagenesis, vol. 26, no. 1, pp. 43–49, 2011.
- H. Stich and M. Rosin, “Micronuclei in exfoliated human cells as an internal dosimeter for exposure to carcinogens,” in Carcinogens and Mutagens in the Environment. Naturally Occurring Compounds, Endogenous Formation and Modulation, H. F. Stich, Ed., vol. 2, pp. 17–25, CRC Press, Boca Raton, Fla, USA, 1983.
- N. K. Mondal, S. Ghosh, and M. R. Ray, “Micronucleus formation and DNA damage in buccal epithelial cells of Indian street boys addicted to gasp 'Golden glue',” Mutation Research, vol. 721, no. 2, pp. 178–183, 2011.
- A. Haveric, S. Haveric, and S. Ibrulj, “Micronuclei frequencies in peripheral blood and buccal exfoliated cells of young smokers and non-smokers,” Toxicology Mechanisms and Methods, vol. 20, no. 5, pp. 260–266, 2010.
- A. Nersesyan, R. Muradyan, M. Kundi, and S. Knasmueller, “Impact of smoking on the frequencies of micronuclei and other nuclear abnormalities in exfoliated oral cells: a comparative study with different cigarette types,” Mutagenesis, vol. 26, no. 2, pp. 295–301, 2011.
- C. Ladeira, S. Viegas, E. Carolino, J. Prista, M. C. Gomes, and M. Brito, “Genotoxicity biomarkers in occupational exposure to formaldehyde: the case of histopathology laboratories,” Mutation Research, vol. 721, no. 1, pp. 15–20, 2011.
- A. Çelik, T. Çavaş, and S. Ergene-Gözükara, “Cytogenetic biomonitoring in petrol station attendants: micronucleus test in exfoliated buccal cells,” Mutagenesis, vol. 18, no. 5, pp. 417–421, 2003.
- A. Celik, S. Yildirim, S. Y. Ekinci, and B. Taşdelen, “Bio-monitoring for the genotoxic assessment in road construction workers as determined by the buccal micronucleus cytome assay,” Ecotoxicology and Environmental Safety, vol. 92, pp. 265–270, 2013.
- P. Rohr, J. da Silva, F. R. da Silva, et al., “Evaluation of genetic damage in open-cast coal mine workers using the buccal micronucleus cytome assay,” Environmental and Molecular Mutagenesis, vol. 54, no. 1, pp. 65–71, 2013.
- S. Pastor, S. Gutiérrez, A. Creus, A. Cebulska-Wasilewska, and R. Marcos, “Micronuclei in peripheral blood lymphocytes and buccal epithelial cells of Polish farmers exposed to pesticides,” Mutation Research, vol. 495, no. 1-2, pp. 147–156, 2001.
- S. Pastor, A. Creus, N. Xamena, C. Siffel, and R. Marcos, “Occupational exposure to pesticides and cytogenetic damage: results of a Hungarian population study using the micronucleus assay in lymphocytes and buccal cells,” Environmental and Molecular Mutagenesis, vol. 40, no. 2, pp. 101–109, 2002.
- S. Burgaz, O. Erdem, G. Čakmak, N. Erdem, A. Karakaya, and A. E. Karakaya, “Cytogenetic analysis of buccal cells from shoe-workers and pathology and anatomy laboratory workers exposed to n-hexane, toluene, methyl ethyl ketone and formaldehyde,” Biomarkers, vol. 7, no. 2, pp. 151–161, 2002.
- S. B. Vuyyuri, M. Ishaq, D. Kuppala, P. Grover, and Y. R. Ahuja, “Evaluation of micronucleus frequencies and DNA damage in glass workers exposed to arsenic,” Environmental and Molecular Mutagenesis, vol. 47, no. 7, pp. 562–570, 2006.
- G. M. D. Bortoli, M. B. D. Azevedo, and L. B. D. Silva, “Cytogenetic biomonitoring of Brazilian workers exposed to pesticides: micronucleus analysis in buccal epithelial cells of soybean growers,” Mutation Research, vol. 675, no. 1-2, pp. 1–4, 2009.
- O. Milosevic-Djordjevic, D. Grujičić, Ž. Vasković, and D. Marinković, “High micronucleus frequency in peripheral blood lymphocytes of untreated cancer patients irrespective of gender, smoking and cancer sites,” Tohoku Journal of Experimental Medicine, vol. 220, no. 2, pp. 115–120, 2010.
- L. N. Rickes, M. C. Alvarengo, T. M. Souza, G. L. Garcias, and M. G. Martino-Roth, “Increased micronucleus frequency in exfoliated cells of the buccal mucosa in hairdressers,” Genetics and Molecular Research, vol. 9, no. 3, pp. 1921–1928, 2010.
- C. Cassini, C. Calloni, G. Bortolini et al., “Occupational risk assessment of oxidative stress and genotoxicity in workers exposed to paints during a working week,” International Journal of Occupational Medicine and Environmental Health, vol. 24, no. 3, pp. 308–319, 2011.
- S. Qayyum, A. Ara, and J. A. Usmani, “Effect of nickel and chromium exposure on buccal cells of electroplaters,” Toxicology and Industrial Health, vol. 28, no. 1, pp. 174–182, 2012.
- C. Bolognesi, “Genotoxicity of pesticides: a review of human biomonitoring studies,” Mutation Research, vol. 543, no. 3, pp. 251–272, 2003.
- S. Bull, K. Fletcher, A. R. Boobis, and J. M. Battershill, “Evidence for genotoxicity of pesticides in pesticide applicators: a review,” Mutagenesis, vol. 21, no. 2, pp. 93–103, 2006.
- H. Schweikl, G. Schmalz, and T. Spruss, “The induction of micronuclei in vitro by unpolymerized resin monomers,” Journal of Dental Research, vol. 80, no. 7, pp. 1615–1620, 2001.
- H. Schweikl, K.-A. Hiller, C. Bolay et al., “Cytotoxic and mutagenic effects of dental composite materials,” Biomaterials, vol. 26, no. 14, pp. 1713–1719, 2005.
- P. Gigola, S. Monarca, D. Feretti, I. Zerbini, and D. D'Argenio, “Evaluation of the clastogenic activity of some resins used in the prosthodontic field,” Minerva stomatologica, vol. 50, no. 11-12, pp. 361–371, 2001.
- U. Heintze, S. Edwardsson, T. Derand, and D. Birkhed, “Methylation of mercury from dental amalgam and mercuric chloride by oral streptococci in vitro,” Scandinavian Journal of Dental Research, vol. 91, no. 2, pp. 150–152, 1983.
- N. Watanabe, H. Kanegane, S. Kinuya et al., “The radiotoxicity of 131I therapy of thyroid cancer: assessment by micronucleus assay of B lymphocytes,” Journal of Nuclear Medicine, vol. 45, no. 4, pp. 608–611, 2004.
- S. Bonassi, L. Hagmar, U. Strömberg et al., “Chromosomal aberrations in lymphocytes predict human cancer independently of exposure to carcinogens,” Cancer Research, vol. 60, no. 6, pp. 1619–1625, 2000.
- S. Beetstra, C. Salisbury, J. Turner et al., “Lymphocytes of BRCA1 and BRCA2 germ-line mutation carriers, with or without breast cancer, are not abnormally sensitive to the chromosome damaging effect of moderate folate deficiency,” Carcinogenesis, vol. 27, no. 3, pp. 517–524, 2006.