Research Article
SeedSeq: Off-Target Transcriptome Database
Table 1
Cause for sequence-dependent off-target effects.
| # | Causes for off-target effects | References |
| 1 | Nearly exact complementarity | [8, 10] | 2 | 15 nt in total or even 11 continuous nt match | [8] | 3 | Seed region complementarity | [9, 12, 15] | 4 | miRNA function (seed region-3″UTR conserved region complementarity) | [13] | 5 | Multiple occurrences of the seed region in an mRNA sequence | [13, 15] | 6 | Complementary region at the cleavage site, center of the siRNA | [11, 16] | 7 | Tolerance of G:U wobble | [16] | 8 | Seed complementation frequency | [12] | 9 | High G/C content in the seed region | [15] |
|
|