SeedSeq: Off-Target Transcriptome Database

Table 1

Cause for sequence-dependent off-target effects.

#	Causes for off-target effects	References
1	Nearly exact complementarity	[8, 10]
2	15 nt in total or even 11 continuous nt match	[8]
3	Seed region complementarity	[9, 12, 15]
4	miRNA function (seed region-3″UTR conserved region complementarity)	[13]
5	Multiple occurrences of the seed region in an mRNA sequence	[13, 15]
6	Complementary region at the cleavage site, center of the siRNA	[11, 16]
7	Tolerance of G:U wobble	[16]
8	Seed complementation frequency	[12]
9	High G/C content in the seed region	[15]