Responses of Growth Performance and Proinflammatory Cytokines Expression to Fish Oil Supplementation in Lactation Sows’ and/or Weaned Piglets’ Diets

Table 2

Oligonucleotide polymerase chain reaction primers.

Gene^1	Accession no.	Primer source	Primer sequences (5^′→3^′)	Orientation	Product size, bp	𝑡𝑎^2 (°C)
IL-1β	M86725	Pig	ATTCGAGTCTGCCCTGTA	Forward	147	54
			TCTGGGTATGGCTTTCCT	Reverse
IL-6	M80258	Pig	GCATTCCCTCCTCTGGTC	Forward	93	58
			ATAGTGTCCTAACGCTCAT	Reverse
TNF-α	AY572787	Pig	CTCCCTCTTTGTCTCCTCC	Forward	77	54
			GCATTGGCATACCCACTCT	Reverse
𝛽-actin	SSU07786	Pig	GGACTTCGAGCAGGAGATGG	Forward	233	—
			GCACCGTGTTGGCGTAGAGG	Reverse

1IL-1β: interleukin 1𝛽, IL-6: interleukin 6, TNF-α: tumor necrosis factor-α.
²𝑡_𝑎: optimal PCR annealing temperature.