Table 1: Immune related, apoptosis-related and tissue destruction genes differentially expressed relative to uninfected cells in monocytes-derived macrophages (MDM), peripheral blood mononuclear cells (PBMC), and in a bovine macrophage cell line after stimulation with live bovine isolates of Map. Genes upregulated or downregulated in at least three of the studies are shown in bold.

Cell modelMap  
strain
Map/cells ratioAssayTime p.i. (h)Upregulated genesDownregulated genes Reference

MDM1ATCC 1969810 : 1qRT-PCR6 hIL-10, GM-CSFIFN-γ, TNF-α [33]
24 hIL-10, GM-CSF
72 hIL-10, GM-CSF, and IL-8IL-12

MDMField strain
B1018
5 : 1qRT-PCR16 hIL-10, TNF-α [34]
24 hIL-10, TNF-α
48 hIL-10TNF-α
96 hIL-10TNF-α

MDMATCC 1969810 : 1Microarray16 hIL-6, TGF-β, TNFR, [35]
Thrombospondin-1, MIP-1β, and MMP-12MHC class II DQ-β

MDMATCC 1969810 : 1Microarray24 hIL-6, TGF-β[36]

PBMC2ATCC 1969810 : 1Microarray16 hIL-1, MMP1, MMP23, and MMP9[37]

MDMATCC 196985 : 1Microarray6 hIL-1β, IL-8, IL-1α, and BCL2A1TNFR, BAD[38]

MDM Field strain 2.1 Microarray2 hIL-10, IL-6, IL-1α, IL-1β, TNF, CXCL2, CCL20, CFB, CASP-1, CASP-4, CASP-8, CASP-6, BIRC-3, CFLAR, and CD40 [39]
6 hIL-1β, TNF, CXCL2, CCL4, CCL5, CCL20, CD40, CFB, CASP-1, CASP4, CASP8, CASP6, BIRC3, and CFLAR
24 hSAA3, CLEC4E, CLEC2D, CD40, and CFB

BoMac3K1010 : 1qRT-PCR4 hIFN-γ, IL-1α, BCL2-1, and TGF-β 1IL-6 [40]
14 hIL-6, BCL2-1
24 hIL-6, TGF-β 1, and TNFα-2MMP3-1, IL-1α, and BCL2-1

MDM: monocytes-derived macrophages; 2PBMC: peripheral blood mononuclear cells; 3BoMac: bovine macrophage cell line.