Mouse Prostate Epithelial Luminal Cells Lineage Originate in the Basal Layer Where the Primitive Stem/Early Progenitor Cells Reside: Implications for Identifying Prostate Cancer Stem Cells
Figure 2
Lineage tracking of keratin 14 expression in developing prostate mouse nodules. (a) Schematic representation of the treatment protocol. (b) β-Galactosidase staining, or fluorescent imaging of the genetic label controlled by basal cell-specific keratin 14 gene promoter activity, showed up in all cell lineages of mouse prostate nodules before and after treatment of the mice with tamoxifen. (c) The more differentiated distal prostate region showed a higher density of β-galactosidase staining than that in the proximal and intermediate regions. The magnification of the microscopy was 400x.
