Mouse Prostate Epithelial Luminal Cells Lineage Originate in the Basal Layer Where the Primitive Stem/Early Progenitor Cells Reside: Implications for Identifying Prostate Cancer Stem Cells
Figure 3
Generation of double transgenic mice keratin 5-tTA: TREtight-H2B-EGFP. (a) The transgenic construct was generated by inserting an H2B-GFP fused gene fragment into the pTRE-Tight vector. (b) Schematic representation of generation of the double-transgenic mice by crossing keratin 5-tTA and TREtight-H2B-EGFP mice. (c) Epifluorescence of a whole animal under UV light. Only the double-transgenic mice with keratin 5-tTA: TREtight-H2B-EGFP showed green skin under UV light.
