Isolation and Characterisation of Mesenchymal Stem Cells from Different Regions of the Human Umbilical Cord

<table>Representative images of (a) chondrogenically-induced cell pellets isolated from different cord regions: cord lining (CL), artery (A), Wharton’s jelly (WJ), vein (V), mixed cord (MC), and bMSC. Scale bar represents 1000 <i>μ</i>m. (b) Osteogenic and adipogenic differentiated cells from MC. (c) Osteogenic and adipogenic differentiated cells from bMSCs. Scale bars represent 100 <i>μ</i>m and 25 <i>μ</i>m for osteogenic and adipogenic images respectively. (d) Scoring of osteogenic and adipogenic differentiation of cells isolated from different regions of the umbilical cord and cells isolated from ileac crest. +++++ denotes scoring system given to the best differentiation seen from bMSCs. <svg height="16.200001" id="M11" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 9.2250004 16.200001" width="9.2250004" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.012,-0,0,-.012,2.9,7.975)"><path d="M409 504l-29 -5q-16 60 -44.5 96t-86.5 36q-54 0 -82.5 -32t-28.5 -77q0 -51 30.5 -82.5t96.5 -65.5l35.5 -18t33 -19.5t33.5 -23.5l27 -25.5t24.5 -32t13.5 -36.5t6 -45q0 -80 -62 -134.5t-160 -54.5q-47 0 -98 15q-22 7 -50 21q-8 23 -27 155l30 7q7 -27 18 -52
t30 -51.5t49 -42.5t67 -16q56 0 88 32.5t32 87.5q0 51 -31.5 82t-98.5 67q-80 44 -110 73q-55 53 -55 124q0 75 56 126.5t150 51.5q53 0 126 -23z" id="x53"></path></g>
</svg>denotes spontaneous differentiation. NT not tested.</table>

BioMed Research International

fig4

Figure 4

Figure 4: Isolation and Characterisation of Mesenchymal Stem Cells from Different Regions of the Human Umbilical Cord 