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BioMed Research International
Volume 2013 (2013), Article ID 916218, 10 pages
Research Article

Synthesis and Antimicrobial Activity of Silver-Doped Hydroxyapatite Nanoparticles

1Department of Multifunctional Materials and Structures Laboratory, National Institute of Materials Physics, 105 Bis Atomistilor, P.O. Box MG 07, 077125 Magurele, Romania
2Faculty of Physics, University of Bucharest, 405 Atomistilor, CP MG-1, 077125 Magurele, Romania
3Microbiology Department, Faculty of Biology, University of Bucharest, Aleea Portocalelor 1-3, 60101 Bucharest, Romania
4EA 4592 Géoressources & Environnement, EGID, Universite Bordeaux, 1 Allée F. Daguin 18, 33607 Pessac Cedex, France

Received 22 September 2012; Revised 15 November 2012; Accepted 15 November 2012

Academic Editor: Abdelwahab Omri

Copyright © 2013 Carmen Steluta Ciobanu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The synthesis of nanosized particles of Ag-doped hydroxyapatite with antibacterial properties is of great interest for the development of new biomedical applications. The aim of this study was the evaluation of (PO4)6(OH)2 nanoparticles (Ag:HAp-NPs) for their antibacterial and antifungal activity. Resistance to antimicrobial agents by pathogenic bacteria has emerged in the recent years and became a major health problem. Here, we report a method for synthesizing Ag doped nanocrystalline hydroxyapatite. A silver-doped nanocrystalline hydroxyapatite was synthesized at 100°C in deionised water. Also, in this paper Ag:HAp-NPs are evaluated for their antimicrobial activity against Gram-positive and Gram-negative bacteria and fungal strains. The specific antimicrobial activity revealed by the qualitative assay is demonstrating that our compounds are interacting differently with the microbial targets, probably due to the differences in the microbial wall structures.