The Cell Surface GRP78 Facilitates the Invasion of Hepatocellular Carcinoma Cells
Figure 3
Forced expression of the ΔKDEL recombinant promotes the adhesion and invasion of hepatocellular carcinoma cells. (a) In-cell western analysis of the cell surface GRP78 in the ΔKDEL transfectants. β-actin served as loading control. sGRP78, cell surface GRP78. (b) Flow cytometry analysis of the cell surface GRP78 in the ΔKDEL transfectants. Red, rabbit isotype IgG. Green, anti-GRP78 antibody. (c) The morphology observation of the ΔKDEL transfectants. The ΔKDEL transfectants were seeded on FN-coated culture dishes, allowed to spread for 2 h. Scale bar, 50 μM. (d) Cell adhesion analysis of the binding ability of the ΔKDEL transfectants with FN-coated substrate. (e) Transwell analysis of the invasive potential of the ΔKDEL transfectants. (Original magnification: 100x.) Data represent the means ± SD of triplicate determinations in three independent experiments. Asterisks indicate that the differences are statistically significant ( versus Mock transfectant; student’s -test).
