The Cell Surface GRP78 Facilitates the Invasion of Hepatocellular Carcinoma Cells

Figure 4

The cell surface GRP78 regulates the activity and secretion of MMP-2 and EMT. (a) Gelatin zymography analysis of the activity of MMP-2 and MMP-9 in Mahlavu and SMMC7721 cells treated with the N-20 antibody. (b) Western blot analysis of the secretion of MMP-2 and MMP-9 in Mahlavu and SMMC7721 cells treated with the N-20 antibody. (c) Gelatin zymography analysis of the activity and secretion of MMP-2 in the ΔKDEL transfectants. (d) Western blot analysis of the expression of E-Cadherin and N-Cadherin in Mahlavu cells treated with the N-20 antibody. (e) Western blot analysis of the expression of E-Cadherin and N-Cadherin in SMMC7721 cells treated with the N-20 antibody. (f) Western blot analysis of the expression of E-Cadherin and N-Cadherin in the ΔKDEL transfectants. UT, untreated. IgG goat isotype IgG. N20, the N20 antibody.