Actin, RhoA, and Rab11 Participation during Encystment in Entamoeba invadens

Figure 8

Rab11 colocalizes with ENO during encystment. Trophozoites were induced to encyst by incubation in LG medium diluted to 47% containing 5% ABS. At 12, 24, 48, 72, and 96 h, aliquots were taken, fixed with 4% paraformaldehyde and blocked with 10% FBS, before staining to analyze the localization of enolase with anti-enolase mAb (1 : 50) (A-5, sc-271384 Santa Cruz Biotechnology), Rab 11 with anti-Rab-11 mAb (1 : 25) (C-19, sc-6565 Santa Cruz Biotechnology), and F-actin with Rhodamine phalloidin (1 : 50) (R415, Invitrogen). Cells were viewed with a Carl Zeiss LSM 700 microscope. Micrographs correspond to 3D optical images. Co-localization, of molecules of interest, is shown by arrows.