Green Tea Polyphenol Epigallocatechin-3-Gallate Enhance Glycogen Synthesis and Inhibit Lipogenesis in Hepatocytes

Figure 4

Effects of GTP-EGCG on expressions of phospho-GSK3β (Ser9) and phospho-GS (Ser641) in HepG2 cells. The cell lysates were separated on 7.5% SDS-PAGE and incubated with antibodies against phospho-GSK3β (Ser9), GSK3β, phospho-GS (Ser641), and GS as described. β-actin was used as a loading control. A representative western blot of phosphorylation of GSK3β and GS from 5 independent experiments is shown. Quantitative data are expressed as mean ± S.E. *𝑃<0.05 and **𝑃<0.01 compared with insulin-stimulated HepG2 cells with 30 mM glucose and ^##𝑃<0.01 compared with HepG2 cells with 5 mM glucose.

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