Molecular Fingerprints to Identify Candida Species

Table 3

Description of SSRs used for MLPs analysis of C. albicans.

	Locus	Ch	Gene product	Repeat motif	Location	DP	Primers	References
1	EF3	5	Elongation factor 3	(TTC)𝑛 (TTTC)𝑛	Upstream region	0.88(1); 0.97(1+3+4); 0.999(ALLexcept2+12)	F: TTTCCTCTTCCTTTCATATAGAA; R: GGATTCACTAGCAGCAGACA	[59, 60, 64, 69, 73]
2	ERK1	4	Extracellular-signal-regulated kinase	(CAGGCT)𝑛(CAAGCT)𝑛; --(CAA)𝑛--(GCCGCA)𝑛; --(CTT)𝑛	Coding region	nr	F: CGACCACGTCATCAATAGAAATCG; R: CGTTGAATGAAACTTGACGAGGGG	[64, 70]
3	CARE-BEME	6	nc	(GAA)𝑛	Noncoding region	0.999(ALLexcept2+12)	F: GAATCATGAAACAGAAACTG; R: TGGGTGAAGGATAATCTGCA	[72, 73]
4	CDC3	1	Cell division cycle protein	(AGTA)𝑛	Downstream region	0.97(1+3+4); 0.999(ALLexcept2+12)	F: CAGATGATTTTTTGTATGAGAAGAA; R: CAGTCACAAGATTAAAATGTTCAAG	[60, 73]
5	HIS3	2	Imidazole glycerol phosphate dehydratase	(ATTT)𝑛	Downstream region	0.97(1+3+4); 0.999(ALLexcept2+12)	F: TGGCAAAAATGATATTCCAA; R: TACACTATGCCCCAAACACA	[60, 73]
6	KRE6	3	𝛽-1,6-Glucan synthesis	(AAT)𝑛	Coding region	0.999(ALLexcept2+12)	F: CAAGCTTATAGTGGCTACTA; R: CCAACACTGATACATCTCG	[64, 73]
7	LOC4; (MRE11)	7	Double-strand break repair protein	(GAA)𝑛	Coding region	0.999(ALLexcept2+12)	F: GTAATGATTACGGCAATGAC; R: AGAACGACGTGTACTATTGG	[64, 73]
8	ZNF1	4	Zinc finger transcription factor	(CAA)𝑛	Coding region	0.999(ALLexcept2+12)	F: CCATTACAGCTGAACCAGCGAGGG; R: CGCTAGGTAACCTACAGATTGTGGC	[64, 73]
9	CAI	4	nc	(CAA)𝑛--(CAA)𝑛	Noncoding region	0.967(9); 0.998(9+10+12); 0.999(ALLexcept2+12)	F: ATGCCATTGAGTGGAATTGG; R: AGTGGCTTGTGTTGGGTTTT	[44, 66, 73]
10	CAIII	5	nc	(GAA)𝑛	Noncoding region	0.853(10); 0.998(9+10+12); 0.999(ALLexcept2+12)	F: TTGGAATCACTTCACCAGGA; R: TTTCCGTGGCATCAGTATCA	[44, 73]
11	CAV	3	nc	(ATT)𝑛	Noncoding region	0.853(11); 0.999(ALLexcept2+12)	F: TGCCAAATCTTGAGATACAAGTG; R: CTTGCTTCTCTTGCTTTAAATTG	[44, 73]
12	CAVI	2	nc	(TAAA)𝑛	Noncoding region	0.853(12); 0.998(9+10+12)	F: ACAATTAAAGAAATGGATTTTAGTCAG; R: TGCTGGTGCTGCTGGTATTA	[44]
13	CAVII	1	nc	(CAAAT)𝑛	Noncoding region	0.670(13); 0.999(ALLexcept2+12)	F: GGGGATAGAAATGGCATCAA; R: TGTGAAACAATTCTCTCCTTGC	[44, 73]

Discriminatory power (DP) based on one or various loci is indicated in brackets according to the row number. Dashed line indicates various nucleotides that separate different microsatellites in the sequence. Ch: chromosome; nc: not corresponding; nr: not reported.