CpG and Interleukin-15 Synergize to Enhance IFN-γ Production by Activated CD8+ T Cells
Figure 5
T cell-derived IFN- is not required for CpG-induced IL-12 production. (a) Thy1.2-depleted splenocytes were isolated from the spleens of naïve (left) or T. cruzi-infected (right) wild-type and Ifng −/− mice on day 9 p.i. Thy1.2-depleted splenocytes were then stimulated with CpG. After 72 hours, culture supernatants were harvested and tested for IL-12p40 by ELISA. (b) Wild-type (filled bars) or Ifng −/− (open bars) CD8+ T cells were purified from the spleens of T. cruzi-infected mice on day 9 p.i. and cocultured with wild-type Thy1.2-depleted APC for 72 hours in the presence of anti-CD3 or anti-CD3+CpG. Cell culture supernatants were then harvested and IL-12p40 levels were determined by ELISA. Results are representative of two independent experiments with two mice per group and are expressed as . NS: not significant.
