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BioMed Research International
Volume 2013 (2013), Article ID 924870, 9 pages
http://dx.doi.org/10.1155/2013/924870
Research Article

Protective Effect of Spin-Labeled 1-Ethyl-1-nitrosourea against Oxidative Stress in Liver Induced by Antitumor Drugs and Radiation

1Department of Chemistry and Biochemistry, Faculty of Medicine, Trakia University, Armeiska Street 11, 6000 Stara Zagora, Bulgaria
2Department of Physiology, Pathophysiology and Pharmacology, Faculty of Medicine, Trakia University, Armeiska Street 11, 6000 Stara Zagora, Bulgaria
3Laboratory of Oncopharmacology, National Cancer Institute, 1756 Sofia, Bulgaria

Received 22 May 2013; Revised 19 August 2013; Accepted 20 August 2013

Academic Editor: Wilson João Cunico Filho

Copyright © 2013 V. Gadjeva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was carried out to investigate possible protection effect of 1-ethyl-3-[4-(2,2,6,6-tetramethylpiperidine-1-oxyl)]-1-nitrosourea (SLENU), synthesized in our laboratory, against oxidative liver injuries induced in mice treated by antitumor drugs: doxorubicin (DOX), bleomycin (BLM), or gamma irradiation (R). Specifically, alterations in some biomarkers of oxidative stress, such as lipid peroxidation products measured as malondialdehyde (MDA) levels and activities of the antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were studied in liver homogenates isolated from tumor bearing C57 black mice after i.p. treatment with solutions of DOX (60 mg/kg), BLM (60 mg/kg), or after total body gamma-irradiation with a single dose of 5 Gy. The same biomarkers were also measured after i.p. pretreatment of mice with SLENU (100 mg/kg). Statistical significant increased MDA levels and SOD and CAT enzymes activities were found in the liver homogenates of tumor bearing mice after alone treatment with DOX or gamma-irradiation compared to the control mice, while these parameters were insignificantly increased after BLM administration compared to the same controls.